Rice gene OsUGT75A regulates seedling emergence under deep-sowing conditions
Jia Zhao,
Siyu Liu,
Xiaoqian Zhao,
Zhibo Huang,
Shan Sun,
Zixuan Zeng,
Yongqi He,
Zhoufei Wang
Affiliations
Jia Zhao
The Laboratory of Seed Science and Technology, Guangdong Key Laboratory of Plant Molecular Breeding, National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, Guangdong, China
Siyu Liu
The Laboratory of Seed Science and Technology, Guangdong Key Laboratory of Plant Molecular Breeding, National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, Guangdong, China
Xiaoqian Zhao
The Laboratory of Seed Science and Technology, Guangdong Key Laboratory of Plant Molecular Breeding, National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, Guangdong, China
Zhibo Huang
The Laboratory of Seed Science and Technology, Guangdong Key Laboratory of Plant Molecular Breeding, National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, Guangdong, China
Shan Sun
The Laboratory of Seed Science and Technology, Guangdong Key Laboratory of Plant Molecular Breeding, National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, Guangdong, China
Zixuan Zeng
The Laboratory of Seed Science and Technology, Guangdong Key Laboratory of Plant Molecular Breeding, National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, Guangdong, China
Yongqi He
Corresponding authors.; The Laboratory of Seed Science and Technology, Guangdong Key Laboratory of Plant Molecular Breeding, National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, Guangdong, China
Zhoufei Wang
Corresponding authors.; The Laboratory of Seed Science and Technology, Guangdong Key Laboratory of Plant Molecular Breeding, National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, Guangdong, China
Poor seedling emergence is a challenge for direct seeding of rice under deep-sowing field conditions. Here we reveal that UDP-glucosyltransferase OsUGT75A promotes rice seedling emergence under deep-sowing conditions by increasing shoot length. Expression of OsUGT75A was higher in the middle regions of the shoot and in shoots under deep-sowing conditions. Levels of free abscisic acid (ABA) and jasmonates (JA) were higher in shoots of OsUGT75A mutants than in those of wild-type plants, and OsUGT75A mutants were more sensitive to ABA and JA treatments. Reduced shoot length was attributed to higher ABA INSENSITIVE 3 (OsABI3) expression and lower JASMONATE-ZIM domain protein (OsJAZ) expression in shoots. Shoot extension by OsUGT75A is achieved mainly by promotion of cell elongation. An elite haplotype of OsUGT75A associated with increased shoot length was identified among indica rice accessions. OsUGT75A acts to increase seedling emergence under deep-sowing conditions.
