Α molecular epidemiological analysis of adenoviruses from excess conjunctivitis cases

BMC Ophthalmology. 2017;17(1):1-7 DOI 10.1186/s12886-017-0447-x

 

Journal Homepage

Journal Title: BMC Ophthalmology

ISSN: 1471-2415 (Online)

Publisher: BMC

LCC Subject Category: Medicine: Ophthalmology

Country of publisher: United Kingdom

Language of fulltext: English

Full-text formats available: PDF, HTML

 

AUTHORS

A. Balasopoulou (Environmental Microbiology Unit, Department of Public Health, Medical School, University of Patras)
P. Κokkinos (Environmental Microbiology Unit, Department of Public Health, Medical School, University of Patras)
D. Pagoulatos (Department of Ophthalmology, Medical School, University of Patras)
P. Plotas (Department of Ophthalmology, Medical School, University of Patras)
O. E. Makri (Department of Ophthalmology, Medical School, University of Patras)
C. D. Georgakopoulos (Department of Ophthalmology, Medical School, University of Patras)
A. Vantarakis (Environmental Microbiology Unit, Department of Public Health, Medical School, University of Patras)

EDITORIAL INFORMATION

Open peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 24 weeks

 

Abstract | Full Text

Abstract Background Τo perform a molecular epidemiological analysis of viral conjunctivitis among excess conjunctivitis cases recorded at the University Hospital of Patras, Greece, for the period March to June 2012. Methods A structured questionnaire containing demographic and clinical data was developed in order to collect retrospective data on the cases. Eye swab specimens were collected and molecular detection of adenoviruses was performed by nested PCR. Positive results were confirmed by sequencing. To determine the relatedness between the isolated sequences, a phylogenetic analysis was conducted. Results The epidemiological analysis (including retrospective data) included 231 conjunctivitis cases (47.1% male, and 52.8% female). Based on clinical features 205 of the cases were diagnosed of viral origin (46.3% male and 53.7% female), 4 of bacterial origin (50% male and 50% female) while 22 were undefined conjunctivitis. The outbreak excess cases (included 156 cases) affected all age groups regardless gender predilection. For the positive samples indicated that 29 samples (72.5%) were AdV17, and 5 (12.5%) as AdV54. Conclusions Molecular analysis could define the cause of viral conjunctivitis, while epidemiological data contributed to the assessment of the risk factors and underlined possible preventive measures. This study is one of the very few on viral conjunctivitis in Greece. This outbreak underscores the need for a national surveillance system for acute infectious conjunctivitis outbreaks. The epidemiological as well as molecular investigation on HAdV ocular infections is rather absent in Greece, which has no surveillance system for viral conjunctivitis.