A Visual Discrimination of Existing States of Virus Capsid Protein by a Giant Molybdate Cluster

Yarong Xue; Mingfen Wei; Dingyi Fu; Yuqing Wu; Bo Sun; Xianghui Yu; Lixin Wu

doi:10.3390/nano12050736

Nanomaterials (Feb 2022)

A Visual Discrimination of Existing States of Virus Capsid Protein by a Giant Molybdate Cluster

Yarong Xue,
Mingfen Wei,
Dingyi Fu,
Yuqing Wu,
Bo Sun,
Xianghui Yu,
Lixin Wu

Affiliations

Yarong Xue: State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, No.2699 Qianjin Street, Changchun 130012, China
Mingfen Wei: State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, No.2699 Qianjin Street, Changchun 130012, China
Dingyi Fu: School of Pharmacy, Nantong University, No. 19 Qixiu Road, Nantong 226001, China
Yuqing Wu: State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, No.2699 Qianjin Street, Changchun 130012, China
Bo Sun: State Engineering Laboratory of AIDS Vaccine, Jilin University, No.2699 Qianjin Street, Changchun 130012, China
Xianghui Yu: State Engineering Laboratory of AIDS Vaccine, Jilin University, No.2699 Qianjin Street, Changchun 130012, China
Lixin Wu: State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, No.2699 Qianjin Street, Changchun 130012, China

DOI: https://doi.org/10.3390/nano12050736
Journal volume & issue: Vol. 12, no. 5
p. 736

Abstract

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We report a unique phenomenon, the opposite color response of a giant polyoxometalate, (NH4)42[Mo132O372(CHCOO)30] (H2O)72 ([Mo132]), to the existing states of human papillomavirus (HPV) major capsid protein, L1-pentamer (L1-p), and virus-like particles (VLPs). The color responses originate from the different assembly forms between [Mo132] and the capsid protein. The latter were inspected and separated by using CsCl gradient centrifugation, and validated in detail by sodium dodecyl sulfate-polyacrylamide gel-electrophoresis (SDS-PAGE), dynamic light scattering (DLS), and transmission electron microscopy (TEM) imaging. Furthermore, the intrinsic mechanisms were investigated in-depth by using XPS-based semi-quantitative analysis and well-designed peptides, revealing the critical points of L1 that determine the charge–transfer ratio between Mo(V) to Mo(VI), and consequently, the levels of [Mo132] hypochromic in different assemblies. Such a unique phenomenon is significant as it supplies a colorimetry approach to distinguish the existing states of the HPV capsid protein and would be significant in the quality assay of the HPV vaccine and existing states of other viruses in the future.

Published in Nanomaterials

ISSN: 2079-4991 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry
Website: https://www.mdpi.com/journal/nanomaterials

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