Invitro Evaluation Of The Effect Of Fetal Hemoglobin Inducer Drugs On Proliferation And Differentiation Of Erythroid Progenitors With β -Thalassemia Gene Mutation
Zahra Kashani Khatib,
Ali Dehghanifard,
Saeid Kaviani,
Mehrdad Noruzinia,
Momeneh Mohammadi,
Fatemeh Mohammad Ali,
Elham Roshandel,
Sahar Mohammadi Fateh,
Shaban Alizadeh
Affiliations
Zahra Kashani Khatib
Master of Sciences Student in Hematology, Hematology Department, School of Medical Sciences, Tarbiat Modares University & Students Scientific Research Center(SSRC), Tehran University of Medical Sciences, Tehran, Iran
Ali Dehghanifard
Ph.D Student in Hematology, Hematology Department, School of Medical Sciences, Tarbiat Modares University and Sarem Cell Research Center (SCRC), Sarem Women’s Hospital, Tehran, Iran
Saeid Kaviani
Associate Professor, Hematology Department, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Mehrdad Noruzinia
Associate Professor, Medical Genetics Department, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Momeneh Mohammadi
Ph.D Student in Hematology, Hematology Department, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Fatemeh Mohammad Ali
Ph.D Student in Hematology, Hematology Department, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Elham Roshandel
Ph.D Student in Hematology, Hematology Department, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Sahar Mohammadi Fateh
Bachelor of Sciences in Medical Laboratory, Sarem Cell Research Center (SCRC), Sarem Women’s Hospital, Tehran, Iran
Shaban Alizadeh
Assistant Professor, Hematology and Transfusion Medicine Department, School of Allied Medicine, Tehran University of Medical Sciences, Tehran, Iran
Background and Aim: Understanding the molecular mechanisms involved in the increased levels of HbF inducing drugs should be advised for effective induction. The aim of this study was to investigate the molecular effects of the drugs thalidomide and sodium butyrate considered as HbF inducer agents. Materials and Methods: In this experimental study, CD133+ cord blood stem cells carrying mutations of heterozygous β-thalassemia were isolated and differentiated into erythroid lineage. In order to evaluate the expression of the erythroid markers, CD71 and CD235a, was analysed. For this purpose, the RNA extracted from erythroid precursors at days 6 and 12 of erythroid differentiation and cDNA synthesized, and then the expression of these genes was performed by quantitative Real-time PCR technique. Results: The results of this study showed the significant effect of thalidomide on erythroid proliferation as compared to sodium butyrate and control group (P<0.05). Also, thalidomide significantly increased CD71expression and decreased CD235a expression as compared to sodium butyrate and control groups (P<0.05). Conclusion : Thalidomide may play its role on HbF induction by increasing the proliferation of early erythroid precursors.
