NUCLEAR REPROGRAMMING AND ITS EPIGENETIC CONSEQUENCES IN SOMATIC CELL NUCLEAR TRANSFER DERIVED ANIMALS
Meirelles FV,
Sampaio RV,
da Silveira JC,
Sangalli JR,
Fantinato-Neto P,
Birgel Jr. E,
Bressan F,
Perecin F
Affiliations
Meirelles FV
Department of Veterinary Medicine, Faculty of Animal Sciences and Food Engineering, University of São Paulo, Brazil; Department of Surgery, Faculty of Veterinary Medicine and Animal Husbandry, University of São Paulo, Brazil.
Sampaio RV
Department of Veterinary Medicine, Faculty of Animal Sciences and Food Engineering, University of São Paulo, Brazil
da Silveira JC
Department of Veterinary Medicine, Faculty of Animal Sciences and Food Engineering, University of São Paulo, Brazil
Sangalli JR
Department of Surgery, Faculty of Veterinary Medicine and Animal Husbandry, University of São Paulo, Brazil
Fantinato-Neto P
Department of Surgery, Faculty of Veterinary Medicine and Animal Husbandry, University of São Paulo, Brazi
Birgel Jr. E
Department of Veterinary Medicine, Faculty of Animal Sciences and Food Engineering, University of São Paulo, Brazil; Department of Surgery, Faculty of Veterinary Medicine and Animal Husbandry, University of São Paulo, Brazil.
Bressan F
Department of Veterinary Medicine, Faculty of Animal Sciences and Food Engineering, University of São Paulo, Brazil; Department of Surgery, Faculty of Veterinary Medicine and Animal Husbandry, University of São Paulo, Brazil.
Perecin F
Department of Veterinary Medicine, Faculty of Animal Sciences and Food Engineering, University of São Paulo, Brazil; Department of Surgery, Faculty of Veterinary Medicine and Animal Husbandry, University of São Paulo, Brazil.
Somatic cell nuclear transfer (SCNT) is a powerful tool to understand the mechanisms driving the cellular reprogramming, as well as addressing fundamental questions on developmental biology. Hundreds of animals have already been generated through this technology, however its large-scale use is still hampered by the low efficiency and disrupted phenotypes reported in SCNT derived newborns. Therefore, this review aims to expose some of the consequences often seen in cloned animals, as well as to discuss and propose some possibilities and perspectives to avoid epigenetic failures during reprogramming.
