International Journal of Molecular Sciences (Jun 2020)
The Strategic Alliance between Clinical and Molecular Science in the War against SARS-CoV-2, with the Rapid-Diagnostics Test as an Indispensable Weapon for Front Line Doctors
- Antonio Vittorino Gaddi,
- Fabio Capello,
- Leonardo Aluigi,
- Pier Luigi Antignani,
- Annapaola Callegaro,
- Gavino Casu,
- Enrico Cipolla,
- Maurizio Cipolla,
- Lucio Cosco,
- Federico Culzoni,
- Francesco Dentali,
- Maria Elexpuru-Zabaleta,
- Tamara Y. Forbes-Hernandez,
- Claudia Fragiacomo,
- Francesca Giampieri,
- Agostino Gnasso,
- Raffaele Mancini,
- Maria Grazia Modena,
- Michele Nichelatti,
- Angelo Virgilio Paradiso,
- Pasquale Ortasi,
- Maria Teresa Savo,
- Flavio Tangianu,
- Sergio Tempesta,
- Tommaso Diego Voci,
- Maurizio Battino
Affiliations
- Antonio Vittorino Gaddi
- Society of Digital Health and Telemedicine, 40100 Bologna, Italy
- Fabio Capello
- International Study Center of Society of Telemedicine and Digital Health, 40100 Bologna, Italy
- Leonardo Aluigi
- Villalba Private Hospital (GVM), 40100 Bologna, Italy
- Pier Luigi Antignani
- Vascular Centre, Nuova Villa Claudia, 00191 Rome, Italy
- Annapaola Callegaro
- Department of Laboratory Medicine ASST-Papa Giovanni XXIII, 24127 Bergamo, Italy
- Gavino Casu
- Cardiology Dept. – ATS Sardegna ASSL, San Francesco Hospital, 08100 Nuoro, Italy
- Enrico Cipolla
- Alma Mater Studiorum Università di Bologna, 40126 Bologna, Italy
- Maurizio Cipolla
- UCCP Catanzaro Lido, ASP Catanzaro; Calabria Society of Telemedicine-Regione Calabria, 88100 Catanzaro, Italy
- Lucio Cosco
- Infectious Disease Department., “Pugliese-Ciaccio” Hospital, 88100 Catanzaro, Italy
- Federico Culzoni
- Culzoni Pharmacy, 20019 Milano, Italy
- Francesco Dentali
- Department of Clinical Medicine Insubria University Varese, 21100 Varese, Italy
- Maria Elexpuru-Zabaleta
- Dipartimento di Scienze Cliniche e Molecolari, Facoltà di Medicina, Università Politecnica delle Marche, 60131 Ancona, Italy
- Tamara Y. Forbes-Hernandez
- Nutrition and Food Science Group, department of Analytical and Food Chemistry, CITACA, CACTI, University of Vigo-Vigo Campus, 32004 Vigo, Spain
- Claudia Fragiacomo
- Moncucco Clinic, 6900 Lugano, Switzerland
- Francesca Giampieri
- Nutrition and Food Science Group, department of Analytical and Food Chemistry, CITACA, CACTI, University of Vigo-Vigo Campus, 32004 Vigo, Spain
- Agostino Gnasso
- Department of Applied Medical Science, Magna Graecia University, 88100 Catanzaro, Italy
- Raffaele Mancini
- Endocrinology and Diabetes Units, ASP, 88100 Catanzaro, Italy
- Maria Grazia Modena
- Surgical, Medical, Dental and Morphological Science Department with Transplantology, Oncological and Rigenerative Address, Modena e Reggio Emilia University, 41125 AOU Modena, Italy
- Michele Nichelatti
- Service of Statistics, Fondazione Malattie del Sangue Niguarda Hospital, 20162 Milano, Italy
- Angelo Virgilio Paradiso
- IRCCS-Istituto Tumori “Giovanni Paolo II”, 70124 Bari, Italy
- Pasquale Ortasi
- Primary health care Department, ASL Area Vasta Romagna, National Medical Interdisciplinary Primary health care Ravenna-Forlì-Cesena, Society of Digital Health and Telemedicine, Emilia Romagna, 48121 Ravenna, Italy
- Maria Teresa Savo
- Dipartimento di Medicina Interna, University of Florence, 50134 Firenze, Italy
- Flavio Tangianu
- ASST Settelaghi Varese, Medical and Surgical Department, Insubria University, 21100 Varese, Italy
- Sergio Tempesta
- Technobios Prenatale Eurogenlab-Caravelli Group, Medical Genetics Laboratory, 40126 Bologna, Italy
- Tommaso Diego Voci
- Associazione Interregionale Cardiologi e Specialisti Medici Ambulatoriali, ACSA, 10125 Torino, Italy
- Maurizio Battino
- Nutrition and Food Science Group, department of Analytical and Food Chemistry, CITACA, CACTI, University of Vigo-Vigo Campus, 32004 Vigo, Spain
- DOI
- https://doi.org/10.3390/ijms21124446
- Journal volume & issue
-
Vol. 21,
no. 12
p. 4446
Abstract
Our work concerns the actual problem of spread of SARS- CoV-2 outbreak which requires fast and correct as possible answer. In current scenario, the need of rapid answer put away the imperative of proper methodology. We focus on the serogical immunoassay for diagnosis of Covid-19 as an important weapon not only for diagnostic purpose, but also for epidemiologic one. The right equilibrium between high speed, low cost and accuracy is obtained with easy-to-use decentralized point-of-care test as the colloidal gold-based immunochromatographic strip assay which detects IgM and IgG antibodies directed against SARS-CoV-2. As our aim is to evaluate the efficacy of Covid-19 rapid tests and of serological assays in real-life settings, we designed a research protocol aimed to establish how to use correctly these diagnostics, taking into account the different possible clinical and epidemiological scenarios.
Keywords
- serological assays
- immunochromatographic rapid test
- colloidal gold rapid test
- sensitivity
- specificity
