EFFECT OF IMMUNOMODULATORS AND ANTI-TULAREMIA VACCINATION ON APOPTOSIS AND LYSIS OF SPLENOCYTES INTERACTING WITH TULYARIN IN VITRO

Abstract

Objective of the study is to evaluate the effect of immunomodulators on the intensity of the post-apoptotic lysis of sensitized organism leukocytes in the presence of specific antigens of tularemia microbe in vitro.Materials and methods. Flow cytometry method was used to determine the relative content of apoptotic and proliferating splenocytes obtained from mice, immunized against tularemia against the background of immunomodulation.Results and conclusions. Obtained is the evidence that is consistent with modern data on the massive leukocyte apoptosis and post-apoptotic leukocyte autolysis (secondary necrosis) in case of tularemia infection. Given the important role of secondary necrosis in the systemic inflammatory response development, the use of immunomodulators suppressing macrophage apoptosis and dead leukocyte lysis, emerging in the course of interaction with Francisella tularensis antigens, may be promising in order to reduce the live tularemia vaccine reactogenicity.

Keywords

• francisella tularensis
• live tularemia vaccine
• apoptosis and secondary necrosis of leukocytes
• polyoxidonium
• dalargin
• flow cytometry