Frontiers in Plant Science (Feb 2021)
AtFUT4 and AtFUT6 Are Arabinofuranose-Specific Fucosyltransferases
- Maria J. Soto,
- Maria J. Soto,
- Maria J. Soto,
- Pradeep Kumar Prabhakar,
- Pradeep Kumar Prabhakar,
- Pradeep Kumar Prabhakar,
- Hsin-Tzu Wang,
- Hsin-Tzu Wang,
- Hsin-Tzu Wang,
- Jason Backe,
- Jason Backe,
- Digantkumar Chapla,
- Digantkumar Chapla,
- Max Bartetzko,
- Ian M. Black,
- Parastoo Azadi,
- Parastoo Azadi,
- Maria J. Peña,
- Maria J. Peña,
- Fabian Pfrengle,
- Fabian Pfrengle,
- Kelley W. Moremen,
- Kelley W. Moremen,
- Breeanna R. Urbanowicz,
- Breeanna R. Urbanowicz,
- Breeanna R. Urbanowicz,
- Michael G. Hahn,
- Michael G. Hahn,
- Michael G. Hahn
Affiliations
- Maria J. Soto
- Lawrence Berkeley National Laboratory, DOE Joint Genome Institute, Berkeley, CA, United States
- Maria J. Soto
- The Complex Carbohydrate Research Center, University of Georgia, Athens, GA, United States
- Maria J. Soto
- Department of Plant Biology, University of Georgia, Athens, GA, United States
- Pradeep Kumar Prabhakar
- The Complex Carbohydrate Research Center, University of Georgia, Athens, GA, United States
- Pradeep Kumar Prabhakar
- Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, United States
- Pradeep Kumar Prabhakar
- Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, United States
- Hsin-Tzu Wang
- The Complex Carbohydrate Research Center, University of Georgia, Athens, GA, United States
- Hsin-Tzu Wang
- Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, United States
- Hsin-Tzu Wang
- Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, United States
- Jason Backe
- The Complex Carbohydrate Research Center, University of Georgia, Athens, GA, United States
- Jason Backe
- Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, United States
- Digantkumar Chapla
- The Complex Carbohydrate Research Center, University of Georgia, Athens, GA, United States
- Digantkumar Chapla
- Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, United States
- Max Bartetzko
- Department of Biomolecular Systems, Max-Planck-Institute of Colloids and Interfaces, Potsdam, Germany
- Ian M. Black
- The Complex Carbohydrate Research Center, University of Georgia, Athens, GA, United States
- Parastoo Azadi
- The Complex Carbohydrate Research Center, University of Georgia, Athens, GA, United States
- Parastoo Azadi
- Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, United States
- Maria J. Peña
- The Complex Carbohydrate Research Center, University of Georgia, Athens, GA, United States
- Maria J. Peña
- Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, United States
- Fabian Pfrengle
- Department of Biomolecular Systems, Max-Planck-Institute of Colloids and Interfaces, Potsdam, Germany
- Fabian Pfrengle
- Department of Chemistry, University of Natural Resources and Life Sciences, Vienna, Austria
- Kelley W. Moremen
- The Complex Carbohydrate Research Center, University of Georgia, Athens, GA, United States
- Kelley W. Moremen
- Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, United States
- Breeanna R. Urbanowicz
- The Complex Carbohydrate Research Center, University of Georgia, Athens, GA, United States
- Breeanna R. Urbanowicz
- Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, United States
- Breeanna R. Urbanowicz
- Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, United States
- Michael G. Hahn
- The Complex Carbohydrate Research Center, University of Georgia, Athens, GA, United States
- Michael G. Hahn
- Department of Plant Biology, University of Georgia, Athens, GA, United States
- Michael G. Hahn
- Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, United States
- DOI
- https://doi.org/10.3389/fpls.2021.589518
- Journal volume & issue
-
Vol. 12
Abstract
The bulk of plant biomass is comprised of plant cell walls, which are complex polymeric networks, composed of diverse polysaccharides, proteins, polyphenolics, and hydroxyproline-rich glycoproteins (HRGPs). Glycosyltransferases (GTs) work together to synthesize the saccharide components of the plant cell wall. The Arabidopsis thaliana fucosyltransferases (FUTs), AtFUT4, and AtFUT6, are members of the plant-specific GT family 37 (GT37). AtFUT4 and AtFUT6 transfer fucose (Fuc) onto arabinose (Ara) residues of arabinogalactan (AG) proteins (AGPs) and have been postulated to be non-redundant AGP-specific FUTs. AtFUT4 and AtFUT6 were recombinantly expressed in mammalian HEK293 cells and purified for biochemical analysis. We report an updated understanding on the specificities of AtFUT4 and AtFUT6 that are involved in the synthesis of wall localized AGPs. Our findings suggest that they are selective enzymes that can utilize various arabinogalactan (AG)-like and non-AG-like oligosaccharide acceptors, and only require a free, terminal arabinofuranose. We also report with GUS promoter-reporter gene studies that AtFUT4 and AtFUT6 gene expression is sub-localized in different parts of developing A. thaliana roots.
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