A new nuclear DNA marker from ubiquitin ligase gene region for genetic diversity detection of walnut germplasm resources

Zhili Suo; Lingna Chen; Dong Pei; Xiaobai Jin; Huijin Zhang

doi:10.1016/j.btre.2014.11.003

Biotechnology Reports (Mar 2015)

A new nuclear DNA marker from ubiquitin ligase gene region for genetic diversity detection of walnut germplasm resources

Zhili Suo,
Lingna Chen,
Dong Pei,
Xiaobai Jin,
Huijin Zhang

Affiliations

Zhili Suo: State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Haidian District, Beijing 100093, China
Lingna Chen: State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Yiheyuan Hou, Beijing 100091, China
Dong Pei: State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Yiheyuan Hou, Beijing 100091, China
Xiaobai Jin: Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Haidian District, Beijing 100093, China
Huijin Zhang: Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Haidian District, Beijing 100093, China

DOI: https://doi.org/10.1016/j.btre.2014.11.003
Journal volume & issue: Vol. 5, no. C
pp. 40 – 45

Abstract

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Development of more sensitive nuclear DNA markers for identification of species, particularly closely allied taxa has been a challenging task that has attracted interest from scientists in fields of biotechnological development and genetic diversity detection. In this study, the sequence of the ubiquitin ligase gene (UBE3) region of nuclear DNA was tested for applicability and efficacy in revealing genetic diversity of walnut resources, with an emphasis on inter- and intra-specific levels. Analysis on genetic relationship among the taxa was conducted with the neighbor-joining (NJ) method. The number of variable bases in the UBE3 region was 20 sites. All nine taxa (species/variety/cultivars) were distinguished using the UBE3 sequence. In addition, each taxon was characterized molecularly with a unique nucleotide molecular formula using ten variable base sites derived from the nuclear DNA UBE3 gene sequence. This study presents a good complementary methodology for developing new DNA markers for identification of genus Juglans.

Published in Biotechnology Reports

ISSN: 2215-017X (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Chemical technology: Biotechnology
Website: https://www.journals.elsevier.com/biotechnology-reports/

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