Kouqiang yixue (Nov 2024)

The inhibitory effect of GsMTx4 on pain sensation during tooth movement in rats

  • WANG Ruofei, SHAO Lixin, LIU Xiaotong, ZHANG Miaomiao

DOI
https://doi.org/10.13591/j.cnki.kqyx.2024.11.003
Journal volume & issue
Vol. 44, no. 11
pp. 815 – 819

Abstract

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Objective To investigate the inhibitory effect of GsMTx4 on pain sensation in rats during tooth movement, open field experiments and immunohistochemical staining by conducting. Methods Fifty-eight male SD rats aged 5-6 weeks were selected and a right maxillary tooth movement model was constructed. The rats were randomly divided into two groups: group A(control group) and group B(GsMTx4 group). They were also divided into 5 subgroups according to 4 h, 1 d, 3 d, 5 d, and 7 d. The left maxillary of the rats in group A was used as group C(blank control group). The open-field test was used to evaluate the pain level of rats. Immunohistochemical staining was used to observe the expression of nerve growth factor(NGF) and calcitonin gene-related peptide(CGRP) in periodontal tissues. Results At 4 h, 1 d, and 3 d, the total distance traveled by rats in group B within 5 minutes in the open-field was higher than that of group A (P<0.05). At 4 h and 1 d, the percentage of time in the center of rats in group B was higher than that in group A(P<0.05). The expressions of NGF and CGRP in groups A and B were higher than those in group C on 1 d, 3 d, 5 d, and 7 d(P<0.05). The expression of NGF and CGRP in group B were lower than those in group A at 4 h, 1 d, and 3 d(P<0.05). Conclusion GsMTx4 can inhibit the expression of NGF and CGRP during tooth movement and relieve the pain caused by tooth movement.

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