Inhibitory Effects of Luteolin 7-Methyl Ether Isolated from <i>Wikstroemia ganpi</i> on Tnf-A/Ifn-Γ Mixture-Induced Inflammation in Human Keratinocyte

Abstract

Plants of the genus Wikstroemia are traditionally used in China to treat various inflammatory diseases. The purpose of this study was to isolate the components of Wikstroemia ganpi (Siebold & Zucc.) Maxim., to evaluate their anti-atopic activities and to identify candidates with anti-atopic therapeutics. A total of 24 compounds were isolated by bioassay-guided separation, including one novel compound, which was tilianin 5-methyl ether. The anti-atopic activities of the isolated compounds were determined using TNF-α-treated RBL-2H3 cells and HaCaT cells. The mRNA expressions of IL-4, IL-6, GM-CSF, G-CSF and TRPV1 were reduced by luteolin 7-methyl ether. The study shows that the luteolin 7-methyl ether isolated from W. ganpi is a potential therapeutic agent for the treatment of atopic dermatitis.

Keywords

• <i>Wikstroemia ganpi</i>
• atopic dermatitis
• tilianin 5-methyl ether
• luteolin 7-methyl ether
• interleukin 4