We report on the optical properties of Bi-doped phosphosilicate fiber. The fiber with a core and a clad diameter of 7.75 µm and 125 µm, respectively, is fabricated in-house using the modified chemical vapor deposition (MCVD) with in-situ solution doping technique. The spectroscopic properties of the fabricated fiber are characterized in terms of absorption, emission and lifetime. The lifetime decay is measured to be 800 µs; indicating a good potential optical amplification in the range of 1300 to 1500 nm. A Bismuth-doped fiber amplifier (BDFA) operating within the O-band region was successfully demonstrated. At 1340 nm, a 14.8 dB gain is achieved with 300 mW pumping power.