REACTIVITY OF RAT ERYTHROCYTES UNDER CONDITIONS OF PHYSICAL STRESS OF DIFFERENT INTENSITY

Abstract

Background. To study the morphofunctional state of red blood in rats depending on the physical load “to failure”. Materials and methods. The study used practically healthy adult male rats of the Wistar: control, staying in water without movement, free swimming and swimming with a load of 2%, 6%, 8%, 10% and 15% of body weight “to failure” according to the protocol on bioethics approved by the IPhys Komi SC UB RAS. In the blood, the level of hemoglobin, hematocrit, the number of erythrocytes and reticulocytes was determined. The diameter of 100 erythrocytes stained according to Romanovsky-Giemsa and diamond cresyl blue was measured. The statistical significance of the differences was assessed using the nonparametric Kruskal-Wallis criterion. Results. The physical load «to failure» was characterized by an increase in hematocrit, hemoglobin, and the number of erythrocytes in the blood of rats and physiological reticulocytosis, along with a decrease in the concentration of hemoglobin in the erythrocyte. It was noted that the distribution of reticulocytes of diameters within 6.4–6.7 μm in floating rats with a weight of 8% of body weight corresponds to the Price-Jones curve of intact animals. Conclusion. In rats, depending on the intensity of exercise, multidirectional shifts in the cellular composition of red blood appeared. Practical use only of the test swimming of rats with a weight of 8% of body weight, judging by the nature of the reaction of erythrocytes, is physiologically justified.

Keywords

• крысы
• эритроциты
• ретикулоциты
• диаметр
• физическая нагрузка «до отказа»