Hyaluronan/Poly-L-lysine/Berberine Nanogels for Impaired Wound Healing
Giovanni Amato,
Maria Aurora Grimaudo,
Carmen Alvarez-Lorenzo,
Angel Concheiro,
Claudia Carbone,
Angela Bonaccorso,
Giovanni Puglisi,
Teresa Musumeci
Affiliations
Giovanni Amato
Department of Drug Sciences, University of Catania; V.le Andrea Doria, 6, 95125 Catania, Italy
Maria Aurora Grimaudo
Department of Drug Sciences, University of Catania; V.le Andrea Doria, 6, 95125 Catania, Italy
Carmen Alvarez-Lorenzo
Departamento de Farmacología, Farmacia y Tecnología Farmacéutica, I+D Farma (GI-1645), Facultad de Farmacia and Health Research Institute of Santiago de Compostela (IDIS), Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain
Angel Concheiro
Departamento de Farmacología, Farmacia y Tecnología Farmacéutica, I+D Farma (GI-1645), Facultad de Farmacia and Health Research Institute of Santiago de Compostela (IDIS), Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain
Claudia Carbone
Department of Drug Sciences, University of Catania; V.le Andrea Doria, 6, 95125 Catania, Italy
Angela Bonaccorso
Department of Drug Sciences, University of Catania; V.le Andrea Doria, 6, 95125 Catania, Italy
Giovanni Puglisi
Department of Drug Sciences, University of Catania; V.le Andrea Doria, 6, 95125 Catania, Italy
Teresa Musumeci
Department of Drug Sciences, University of Catania; V.le Andrea Doria, 6, 95125 Catania, Italy
Physiological wound healing process can be delayed in the presence of certain pathologies, such as diabetes or cancer. In this perspective, the aim of this study was to design a new nanogel platform of hyaluronan, poly-L-lysine and berberine suitable for wound treatment. Two different nanogel formulations were selected after a first formulation screening. They were prepared by adding dropwise 2 mg/mL hyaluronan aqueous solution (200 or 700 kDa) to 1.25 mg/mL poly-L-lysine aqueous solution. Blank nanogels formulated with 200 kDa HA resulted stable after freeze-drying with dimensions, polydispersity index and zeta potential of 263.6 ± 13.1 nm, 0.323 ± 0.029 and 32.7 ± 3.5 mV, respectively. Both blank and berberine-loaded nanogels showed rounded-shape structures. Loaded nanogels released nearly 50% of loaded berberine within 45 min, whereas the remaining 50% was released up to 24 h in vitro. Both, blank and berberine-loaded nanogels were able to completely close the fibroblasts gap in 42 h.
