Effects of ω-3 PUFA and ascorbic acid combination on post-resuscitation myocardial function

Cheng Cheng; Hui Li; Lian Liang; Tao Jin; Guozhen Zhang; Jennifer L. Bradley; Mary Ann Peberdy; Joseph P. Ornato; Dayanjan S. Wijesinghe; Wanchun Tang

Biomedicine & Pharmacotherapy (Jan 2021)

Effects of ω-3 PUFA and ascorbic acid combination on post-resuscitation myocardial function

Cheng Cheng,
Hui Li,
Lian Liang,
Tao Jin,
Guozhen Zhang,
Jennifer L. Bradley,
Mary Ann Peberdy,
Joseph P. Ornato,
Dayanjan S. Wijesinghe,
Wanchun Tang

Affiliations

Cheng Cheng: Department of Cardiology, The Second Hospital of Anhui Medical University, Hefei, China; Weil Institute of Emergency and Critical Care Research, Virginia Commonwealth University, Richmond, VA, USA
Hui Li: Weil Institute of Emergency and Critical Care Research, Virginia Commonwealth University, Richmond, VA, USA; Department of Intensive Care Unit, The Second Hospital of Anhui Medical University, Hefei, China
Lian Liang: Weil Institute of Emergency and Critical Care Research, Virginia Commonwealth University, Richmond, VA, USA
Tao Jin: Weil Institute of Emergency and Critical Care Research, Virginia Commonwealth University, Richmond, VA, USA
Guozhen Zhang: Weil Institute of Emergency and Critical Care Research, Virginia Commonwealth University, Richmond, VA, USA
Jennifer L. Bradley: Weil Institute of Emergency and Critical Care Research, Virginia Commonwealth University, Richmond, VA, USA
Mary Ann Peberdy: Weil Institute of Emergency and Critical Care Research, Virginia Commonwealth University, Richmond, VA, USA; Departments of Internal Medicine and Emergency Medicine, Virginia Commonwealth University Health System, Richmond, VA, USA
Joseph P. Ornato: Weil Institute of Emergency and Critical Care Research, Virginia Commonwealth University, Richmond, VA, USA; Department of Emergency Medicine, Virginia Commonwealth University Health System, Richmond, VA, USA
Dayanjan S. Wijesinghe: School of Pharmacy, Virginia Commonwealth University, Richmond, VA, USA; Institute for Structural Biology, Drug Discovery and Development, Virginia Commonwealth University, Richmond, VA, USA; Da Vinci Center, Virginia Commonwealth University, Richmond, VA, USA; Corresponding author at: School of Pharmacy, Virginia Commonwealth University, Richmond, VA, USA.
Wanchun Tang: Weil Institute of Emergency and Critical Care Research, Virginia Commonwealth University, Richmond, VA, USA; Department of Emergency Medicine, Virginia Commonwealth University Health System, Richmond, VA, USA; Corresponding author at: Weil Institute of Emergency and Critical Care Research at VCU, Box 980266, Sanger Hall, 1101 E Marshall St, Richmond, VA, 23298-0279, USA.

Journal volume & issue: Vol. 133
p. 110970

Abstract

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Accumulating evidence demonstrated that administration of ω-3 polyunsaturated fatty acid (ω-3 PUFA) or ascorbic acid (AA) following cardiac arrest (CA) improves survival. Therefore, we investigate the effects of ω-3 PUFA combined with AA on myocardial function after CA and cardiopulmonary resuscitation (CPR) in a rat model.Thirty male rats were randomized into 5 groups: (1) sham; (2) control; (3) ω-3 PUFA; (4) AA; (5) ω-3 PUFA + AA. Ventricular fibrillation (VF) was induced and untreated for 6 min followed by defibrillation after 8 min of CPR. Infusion of drug or vehicle occurred at the start of CPR. Myocardial function and sublingual microcirculation were measured at baseline and after return of spontaneous circulation (ROSC). Heart tissues and blood were collected 6 h after ROSC. Myocardial function and sublingual microcirculation improvements were seen with ω-3 PUFA or AA compared to control after ROSC (p < 0.05). ω-3 PUFA + AA shows a better myocardial function than ω-3 PUFA or AA (p < 0.05). ω-3 PUFA or AA decreases pro-inflammatory cytokines, cTnI, myocardium malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) modified proteins compared to control (p < 0.05). ω-3 PUFA and AA combined have lower MDA and 4-HNE modified proteins than alone (p < 0.05). ω-3 PUFA or AA treatment reduces the severity of post-resuscitation myocardial dysfunction, improves sublingual microcirculation, decreases lipid peroxidation and systemic inflammation in the early phase of recovery following CA and resuscitation. A combination of ω-3 PUFA and AA treatment confers an additive effect in suppressing lipid peroxidation and improving myocardial function.

Published in Biomedicine & Pharmacotherapy

ISSN: 0753-3322 (Print); 1950-6007 (Online)
Publisher: Elsevier
Country of publisher: France
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: https://www.journals.elsevier.com/biomedicine-and-pharmacotherapy

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