IN VITRO ACTION OF THE DRUG CANDIDATE OF PBTZ169, HYDROCHLORIDE ACTION IN RESPECT OF CLINICAL STRAINS OF MYCOBACTERIUM TUBERCULOSIS WITH EXTENSIVE DRUG RESISTANCE

Abstract

Goal of the study: to study the specific in vitro action of PBTZ169, hydrochloride, in respect of clinical strains of M. tuberculosis with extensive drug resistance (XDR).Materials and methods. Strains of M. tuberculosis isolated from 20 XDR tuberculosis patients (10 strains from HIV positive patients and 10 strains from HIV negative patients) were used in the study, their susceptibility to 10 anti-tuberculosis first and second lines drugs plus linezolid was tested by Bactec MGIT 960. Bacteriostatic and bactericidal action of PBTZ169, hydrochloride, was defined as per the growth speed of M. tuberculosis strains in Bactec MGIT 960 compared to the growth of strains on the medium free from drugs and the medium containing control drugs (INH 0.1 мcg/ml, RIF 1 мcg/ml, AMK 1 мcg/ml and LFX 1.5 мcg/ml).Results. The used clinical strains of Mycobacterium tuberculosis were resistant to 7-10 anti-tuberculosis drugs, of them 3 were resistant to linezolid. When testing bacteriostatic action it was found that PBTZ169, hydrochloride, in the concentration of 0.037 mcg/ml fully suppressed growth of all 10 strains isolated from HIV negative patients and of the majority of strains isolated from HIV positive tuberculosis patients (7 out of 10). In the same concentration PBTZ169HCl demonstrated bactericidal action in respect of all strains isolated from HIV negative tuberculosis patients and 4 out of 10 strains isolated from HIV positive tuberculosis patients. Forthe remaining 6 out of 10 strains from HIV positive tuberculosis patients minimum bactericide concentration made 0.111 mcg/ml.

Keywords

• мбт с шлу
• мик
• мбк