Nature Communications (Nov 2018)
Potassium channel-based optogenetic silencing
- Yinth Andrea Bernal Sierra,
- Benjamin R. Rost,
- Martin Pofahl,
- António Miguel Fernandes,
- Ramona A. Kopton,
- Sylvain Moser,
- Dominik Holtkamp,
- Nicola Masala,
- Prateep Beed,
- John J. Tukker,
- Silvia Oldani,
- Wolfgang Bönigk,
- Peter Kohl,
- Herwig Baier,
- Franziska Schneider-Warme,
- Peter Hegemann,
- Heinz Beck,
- Reinhard Seifert,
- Dietmar Schmitz
Affiliations
- Yinth Andrea Bernal Sierra
- Institute of Biology, Experimental Biophysics, Humboldt-Universität zu Berlin
- Benjamin R. Rost
- Charité-Universitätsmedizin Berlin, Corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Neuroscience Research Center
- Martin Pofahl
- Institute for Experimental Epileptology and Cognition Research, University of Bonn Medical Center
- António Miguel Fernandes
- Department Genes–Circuits–Behavior, Max Planck Institute of Neurobiology
- Ramona A. Kopton
- Institute for Experimental Cardiovascular Medicine, University Heart Center, Medical Center – University of Freiburg, and Faculty of Medicine, University of Freiburg
- Sylvain Moser
- Department Genes–Circuits–Behavior, Max Planck Institute of Neurobiology
- Dominik Holtkamp
- Institute for Experimental Epileptology and Cognition Research, University of Bonn Medical Center
- Nicola Masala
- Institute for Experimental Epileptology and Cognition Research, University of Bonn Medical Center
- Prateep Beed
- Charité-Universitätsmedizin Berlin, Corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Neuroscience Research Center
- John J. Tukker
- Charité-Universitätsmedizin Berlin, Corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Neuroscience Research Center
- Silvia Oldani
- Charité-Universitätsmedizin Berlin, Corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Neuroscience Research Center
- Wolfgang Bönigk
- Molecular Sensory Systems, Center of Advanced European Studies and Research (caesar)
- Peter Kohl
- Institute for Experimental Cardiovascular Medicine, University Heart Center, Medical Center – University of Freiburg, and Faculty of Medicine, University of Freiburg
- Herwig Baier
- Department Genes–Circuits–Behavior, Max Planck Institute of Neurobiology
- Franziska Schneider-Warme
- Institute for Experimental Cardiovascular Medicine, University Heart Center, Medical Center – University of Freiburg, and Faculty of Medicine, University of Freiburg
- Peter Hegemann
- Institute of Biology, Experimental Biophysics, Humboldt-Universität zu Berlin
- Heinz Beck
- Institute for Experimental Epileptology and Cognition Research, University of Bonn Medical Center
- Reinhard Seifert
- Molecular Sensory Systems, Center of Advanced European Studies and Research (caesar)
- Dietmar Schmitz
- Charité-Universitätsmedizin Berlin, Corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Neuroscience Research Center
- DOI
- https://doi.org/10.1038/s41467-018-07038-8
- Journal volume & issue
-
Vol. 9,
no. 1
pp. 1 – 13
Abstract
Optogenetic tools enable precise experimental control of the behaviour of cells. Here, the authors introduce a genetically-encoded two-protein system that enables silencing of excitable cells such as neurons and cardiomyocytes using blue light, and demonstrate its utility both in vitro and In vivo.
