Protocol for imaging proteins associated with Legionella-containing vacuoles in host cells

Abstract

Summary: The intracellular bacterial pathogen Legionella pneumophila exploits host cellular systems using approximately 300 effector proteins to establish a replicative niche known as the Legionella-containing vacuole (LCV). During infection, both host and bacterial proteins interactively function on the LCVs. Here, we describe a detailed step-by-step protocol to visualize proteins associated with LCVs in host cells. This protocol can aid in analyzing whether a protein of interest influences the subcellular localization of LCV-associated proteins during infection.For complete details on the use and execution of this protocol, please refer to Kitao et al. (2020).

Keywords

• Cell-based Assays
• Immunology
• Microscopy