Inhibitory effect of polysaccharides extracted from Changbai Mountain Ganoderma lucidum on periodontal inflammation
Zhen Chen,
Wenguang Qin,
Hongbing Lin,
Yang Liu,
Yue Tian,
Xuetao Zhao,
Tong Ding,
Yawei Wang,
Tianjiao Mao,
Jiang Li,
Yuqin Shen
Affiliations
Zhen Chen
Department of Periodontics, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, Guangdong, 510182, China
Wenguang Qin
Department of Periodontics, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, Guangdong, 510182, China
Hongbing Lin
Department of Periodontics, School of Stomatology, Jilin University, Changchun, 130021, Jilin Province, China
Yang Liu
The Engineering Research Centre of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, Jilin, 130018, China
Yue Tian
Department of Periodontics, School of Stomatology, Jilin University, Changchun, 130021, Jilin Province, China
Xuetao Zhao
Department of Periodontics, School of Stomatology, Jilin University, Changchun, 130021, Jilin Province, China
Tong Ding
Department of Periodontics, School of Stomatology, Jilin University, Changchun, 130021, Jilin Province, China
Yawei Wang
Department of Periodontics, School of Stomatology, Jilin University, Changchun, 130021, Jilin Province, China
Tianjiao Mao
Department of Periodontics, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, Guangdong, 510182, China
Jiang Li
Department of Periodontics, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, Guangdong, 510182, China; Corresponding author.
Yuqin Shen
Department of Periodontics, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, Guangdong, 510182, China; Corresponding author.
As the main bioactive substance of Ganoderma lucidum, Ganoderma lucidum polysaccharide (GLP) has anti-inflammatory, antibacterial, and other biological activities. Studies have shown that GLP can regulate the expression of multiple inflammatory cytokines in different inflammatory models and diseases as part of the anti-infection immune response. We extracted crude Changbai Mountain Ganoderma lucidum polysaccharides (CGLPs), analyzed their physical and chemical properties, and then applied them to the periodontitis model to verify whether they have an inhibitory effect on mouse periodontitis. CGLP was determined to be a heteropolysaccharide with dextran as the main component. Its molecular weight was 17.40 kDa. In vivo experiments in mice showed that CGLP can inhibit the alveolar bone loss and reduced inflammation caused of periodontitis by regulating the expression of the inflammatory factors IL-1β, TNF-α, and IL-10 in a concentration-dependent manner.
