Frontiers in Microbiology (Jun 2022)
Riboflavin Attenuates Influenza Virus Through Cytokine-Mediated Effects on the Diversity of the Gut Microbiota in MAIT Cell Deficiency Mice
- Ying Li,
- Ying Li,
- Ying Li,
- Ying Li,
- Chun-Wei Shi,
- Chun-Wei Shi,
- Chun-Wei Shi,
- Chun-Wei Shi,
- Yu-Ting Zhang,
- Yu-Ting Zhang,
- Yu-Ting Zhang,
- Yu-Ting Zhang,
- Hai-Bin Huang,
- Hai-Bin Huang,
- Hai-Bin Huang,
- Hai-Bin Huang,
- Yan-Long Jiang,
- Yan-Long Jiang,
- Yan-Long Jiang,
- Yan-Long Jiang,
- Jian-Zhong Wang,
- Jian-Zhong Wang,
- Jian-Zhong Wang,
- Jian-Zhong Wang,
- Xin Cao,
- Xin Cao,
- Xin Cao,
- Xin Cao,
- Nan Wang,
- Nan Wang,
- Nan Wang,
- Nan Wang,
- Yan Zeng,
- Yan Zeng,
- Yan Zeng,
- Yan Zeng,
- Gui-Lian Yang,
- Gui-Lian Yang,
- Gui-Lian Yang,
- Gui-Lian Yang,
- Wen-Tao Yang,
- Wen-Tao Yang,
- Wen-Tao Yang,
- Wen-Tao Yang,
- Chun-Feng Wang,
- Chun-Feng Wang,
- Chun-Feng Wang,
- Chun-Feng Wang
Affiliations
- Ying Li
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Ying Li
- Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Ying Li
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Ying Li
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Chun-Wei Shi
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Chun-Wei Shi
- Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Chun-Wei Shi
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Chun-Wei Shi
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Yu-Ting Zhang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Yu-Ting Zhang
- Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Yu-Ting Zhang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Yu-Ting Zhang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Hai-Bin Huang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Hai-Bin Huang
- Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Hai-Bin Huang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Hai-Bin Huang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Yan-Long Jiang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Yan-Long Jiang
- Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Yan-Long Jiang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Yan-Long Jiang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Jian-Zhong Wang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Jian-Zhong Wang
- Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Jian-Zhong Wang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Jian-Zhong Wang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Xin Cao
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Xin Cao
- Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Xin Cao
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Xin Cao
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Nan Wang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Nan Wang
- Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Nan Wang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Nan Wang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Yan Zeng
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Yan Zeng
- Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Yan Zeng
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Yan Zeng
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Gui-Lian Yang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Gui-Lian Yang
- Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Gui-Lian Yang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Gui-Lian Yang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Wen-Tao Yang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Wen-Tao Yang
- Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Wen-Tao Yang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Wen-Tao Yang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- Chun-Feng Wang
- College of Veterinary Medicine, Jilin Agricultural University, Changchun, China
- Chun-Feng Wang
- Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Agricultural University, Changchun, China
- Chun-Feng Wang
- Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, China
- Chun-Feng Wang
- Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China
- DOI
- https://doi.org/10.3389/fmicb.2022.916580
- Journal volume & issue
-
Vol. 13
Abstract
Influenza is a serious respiratory disease that continues to threaten global health. Mucosa-associated invariant T (MAIT) cells use T-cell receptors (TCRs) that recognize microbial riboflavin derived intermediates presented by the major histocompatibility complex (MHC) class I-like protein MR1. Riboflavin synthesis is broadly conserved, but the roles or mechanisms of riboflavin in MR1–/– mouse influenza infection are not well understood. In our study, immunofluorescence techniques were applied to analyze the number and distribution of viruses in lung tissue. The amount of cytokine expression was assessed by flow cytometry (FCM), ELISA, and qPCR. The changes in the fecal flora of mice were evaluated based on amplicon sequencing of the 16S V3-V4 region. Our study showed that MAIT cell deficiency increased mortality and that riboflavin altered these effects in microbiota-depleted mice. The oral administration of riboflavin inhibited IL-1β, IL-17A, and IL-18 production but significantly increased the expression of IFN-γ, TNF-α, CCL2, CCL3, and CCL4 in a mouse model. The analysis of the mouse flora revealed that riboflavin treatment significantly increased the relative abundance of Akkermansia and Lactobacillus (p < 0.05) and decreased that of Bacteroides. In contrast, MR1–/– mice exhibited a concentrated aggregation of Bacteroides (p < 0.01), which indicated that MAIT cell deficiency reduced the diversity of the bacterial population. Our results define the functions of MAIT cells and riboflavin in resistance to influenza virus and suggest a potential role for riboflavin in enhancing MAIT cell immunity and the intestinal flora diversity. Gut populations can be expanded to enhance host resistance to influenza, and the results indicate novel interactions among viruses, MAIT cells, and the gut microbiota.
Keywords
