Assessment of Drought Tolerance Indices and their Relation with ISSR Markers in Bread Wheat (Triticum aestivum L.)

Abstract

Water stress is one of the most important environmental abiotic stress that reduced crop yield especially in arid and semi arid regions of the world. In order to identifying drought tolerance, 39 cultivars of spring, facultative and winter type wheat varieties were planted as subplots within the irrigation plots (main plots) in a randomized complete block design with three replications in a research filed of Faculty of Agriculture, University of Mohaghegh Ardabili, Iran. In 2/3 continuous irrigation stress level, based on stress susceptibility, geometric mean productivity and harmonic indices, Sepahan, Karaj 3, Bahar and Yavaroos were known as the best varieties; and in 1/3 continuous irrigation stress level, based on the mentioned indices, Hirmand, Bahar, Yavaroos and Marvdasht were the best one. According to biplot resulted from principle coordinate analysis, in 2/3 continuous irrigation Moghan 1, Golestan, Kavir, Maroon, Karkheh, Chanaab, 10, 6, Bahar and Sepahan; and in 1/3 continuous irrigation Golestan, 10, Niknejad, Maroon, Darab, Falat, Arta, Marvdasht, Bahar and Hirmand were identified as the most tolerant cultivars. According to multiple regression analysis in subject of agronomic traits, 43, 33 and 25 informative ISSR markers identified in control, 2/3 and 1/3 continuous irrigation conditions, respectively. Also among these markers, there were significant relationship between P12L3 and P21L3 markers with plant height and spike length; P4L1 and P22L1 markers with flag leaf length; P19L4 markers and number of node; P30L4 markers and awn length; P10L1 and P22L1 with peduncle to plant height ratio in all of stress and non-stress conditions.