Cancer Management and Research (Oct 2020)
Knockdown of circ-TTBK2 Inhibits Glioma Progression by Regulating miR-1283 and CHD1
Abstract
Chengchen Han, Shuwei Wang, Hongwei Wang, Jianning Zhang Department of Neurosurgery, The Sixth Medical Center of PLA General Hospital, Beijing 100048, People’s Republic of ChinaCorrespondence: Jianning ZhangDepartment of Neurosurgery, The Sixth Medical Center of PLA General Hospital, No. 6, Fucheng Road, Haidian District, Beijing, People’s Republic of ChinaTel +86 10-66958114Email [email protected]: Dysregulated circular RNAs (circRNAs) are involved in the development of glioma. This paper aims to analyze the role and mechanism of circRNA tau tubulin kinase 2 (circ-TTBK2) in glioma progression.Methods: The glioma samples and normal brain tissues were collected. The levels of circ-TTBK2, microRNA-1283 (miR-1283) and chromodomain helicase DNA-binding protein 1 (CHD1) were examined via quantitative reverse transcription polymerase chain reaction or Western blot. Cell proliferation, migration, invasion and glycolysis were determined via 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide, transwell assay, Western blot, glucose and lactate assay kits. The target relationship was analyzed via dual-luciferase reporter assay. The xenograft model was established using U251 cells.Results: circ-TTBK2 expression was increased in glioma tissues and cells. circ-TTBK2 knockdown suppressed glioma cell proliferation, migration, invasion and glycolysis. circ-TTBK2 was a sponge for miR-1283, and knockdown of miR-1283 reversed the effect of circ-TTBK2 silence on glioma progression. CHD1 was targeted via miR-1283, and miR-1283 repressed glioma cell proliferation, migration, invasion and glycolysis via decreasing CHD1. Knockdown of circ-TTBK2-reduced CHD1 expression by mediating miR-1283. Silence of circ-TTBK2 reduced xenograft tumor growth.Conclusion: Down-regulation of circ-TTBK2 suppressed glioma development by regulating miR-1283 and CHD1, providing a new mechanism for understanding glioma pathogenesis.Keywords: glioma, circ-TTBK2, miR-1283, CHD1, migration, glycolysis
