Quantitative detection of enrofloxacin in chicken and pork by magnetic solid phase extraction and surface-enhanced Raman spectroscopy

Abstract

ObjectiveTo develop a rapid and quantitative method for the detection of enrofloxacin in chicken and pork using magnetic solid phase extraction （MSPE） combined with surface enhanced Raman spectroscopy （SERS）.MethodsMagnetic Fe3O4@ covalent organic framework （COF） nanocomposites were synthesized and applied as absorbent to separate and enrich enrofloxacin. The SERS spectra of enrofloxacin were collected by a portable Raman spectrometer with Ag nanoparticles as enhancing substrate. The characteristic SERS signals of enrofloxacin were used to realize its quantification.ResultsIntensities of SERS signals at 745.77 cm-1 and the logarithmic values of the concentrations of enrofloxacin in the range of 5.0×10-7 to 1.0×10-5 mol/L displayed a good linear relationship with a coefficient of determination of 0.962. The limit of detection and limit of quantitation were 0.07 and 0.23 µg/g respectively. The recoveries of enrofloxacin in chicken and pork were 80.97%~100.98% with relative standard deviations of 1.6%~4.6%.ConclusionThe developed method has the advantages of simple in operate， short in time， high in sensitivity and stability， which provides a new method for the rapid detection and on-site detection of enrofloxacin.

Keywords

• enrofloxacin
• rapid detection
• magnetic solid phase extraction
• surface enhanced raman spectroscopy
• antibiotic
• residue of veterinary drug