International Journal of Nanomedicine (Dec 2021)

Protective Effects of Chitosan-Bilirubin Nanoparticles Against Ethanol-Induced Gastric Ulcers

  • Huang Z,
  • Shi Y,
  • Wang H,
  • Chun CJ,
  • Chen L,
  • Wang K,
  • Lu Z,
  • Zhao Y,
  • Li X

Journal volume & issue
Vol. Volume 16
pp. 8235 – 8250

Abstract

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Zhiwei Huang,1– 3,* Yannan Shi,2,* Hengcai Wang,2,* Changju Chun,3 Longwang Chen,1,4 Kang Wang,1,4 Zhongqiu Lu,1,4 Yingzheng Zhao,2 Xinze Li1,4 1Department of Emergency, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325035, People’s Republic of China; 2School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035, People’s Republic of China; 3Research Institute of Pharmaceutical Sciences, College of Pharmacy, Chonnam National University, Gwangju, 61186, Republic of Korea; 4Wenzhou Key Laboratory of Emergency and Disaster Medicine, Wenzhou, 325035, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xinze Li; Yingzheng ZhaoWenzhou Medical University, University Town, Chashan, Wenzhou, Zhejiang, 325035, People’s Republic of ChinaEmail [email protected]; [email protected]: Gastric ulcers (GU) are a disease of the gastrointestinal tract that can be caused by excessive alcohol consumption and heavy use of nonsteroidal anti-inflammatory drugs. GU manifests predominantly as pathological damage, such as extensive inflammatory erosion and superficial bleeding of the gastric mucosa. Oxidative stress damage and the inflammatory response are now considered important predisposing factors for GU, suggesting that antioxidant and anti-inflammatory drugs could be treatments for GU. Nanoparticle drug carriers offer many advantages over conventional drugs, such as improved drug efficiency, increased drug stability, and increased half-life.Methods: We designed chitosan-bilirubin conjugate (CS-BR) nanoparticles and assessed the anti-inflammatory and antioxidant abilities of CS-BR in gastric epithelial cells. Then, we evaluated the intragastric retention time and the anti-ulcer effects of CS-BR in vivo.Results: The in vitro data showed that CS-BR nanoparticles protect gastric epithelial cells against oxidative/inflammatory injury. The in vivo study demonstrated that CS-BR nanoparticles accumulate permanently in the stomach and exert powerful antioxidant and anti-inflammatory effects against GU.Conclusion: This study applied bilirubin to the treatment of GU and confirmed that CS-BR nanoparticles are effective at alleviating acute GU in an experimental model. The findings provide innovative ideas for prophylaxis against or treatment of GU.Keywords: oxidative stress, inflammation, reactive oxygen species, gastroprotection

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