Nature Communications (Jan 2020)

Chaperone mediated detection of small molecule target binding in cells

  • Kelvin F. Cho,
  • Taylur P. Ma,
  • Christopher M. Rose,
  • Donald S. Kirkpatrick,
  • Kebing Yu,
  • Robert A. Blake

DOI
https://doi.org/10.1038/s41467-019-14033-0
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 11

Abstract

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Quantitative profiling of small molecule-protein binding in cells can aid basic biochemical research and drug discovery. Here, the authors develop the Heat Shock Protein Inhibition Protein Stability Assay (HIPStA) as a high-throughput method to assess cellular target engagement and identify new drug targets.