Protective Effects of Topical Administration of Laminarin in Oxazolone-Induced Atopic Dermatitis-like Skin Lesions
Tae-Kyeong Lee,
Dae Won Kim,
Ji Hyeon Ahn,
Choong-Hyun Lee,
Jae-Chul Lee,
Soon Sung Lim,
Il Jun Kang,
Seongkweon Hong,
Soo Young Choi,
Moo-Ho Won,
Joon Ha Park
Affiliations
Tae-Kyeong Lee
Department of Food Science and Nutrition, Hallym University, Chuncheon 24252, Gangwon, Korea
Dae Won Kim
Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Gangnung-Wonju National University, Gangneung 25457, Gangwon, Korea
Ji Hyeon Ahn
Department of Physical Therapy, College of Health Science, Youngsan University, Yangsan 50510, Gyeongnam, Korea
Choong-Hyun Lee
Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan 31116, Chungbuk, Korea
Jae-Chul Lee
Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 24341, Gangwon, Korea
Soon Sung Lim
Department of Food Science and Nutrition, Hallym University, Chuncheon 24252, Gangwon, Korea
Il Jun Kang
Department of Food Science and Nutrition, Hallym University, Chuncheon 24252, Gangwon, Korea
Seongkweon Hong
Department of Surgery, Kangwon National University Hospital, School of Medicine, Kangwon National University, Chuncheon 24289, Gangwon, Korea
Soo Young Choi
Department of Biomedical Science, Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon 24252, Gangwon, Korea
Moo-Ho Won
Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 24341, Gangwon, Korea
Joon Ha Park
Department of Anatomy, College of Korean Medicine, Dongguk University, Gyeongju 38066, Gyeongbuk, Korea
Laminarin is a polysaccharide isolated from brown marine algae and has a wide range of bioactivities, including immunoregulatory and anti-inflammatory properties. However, the effects of laminarin on atopic dermatitis have not been demonstrated. This study investigated the potential effects of topical administration of laminarin using a Balb/c mouse model of oxazolone-induced atopic dermatitis-like skin lesions. Our results showed that topical administration of laminarin to the ear of the mice improved the severity of the dermatitis, including swelling. Histological analysis revealed that topical laminarin significantly decreased the thickening of the epidermis and dermis and the infiltration of mast cells in the skin lesion. Serum immunoglobulin E levels were also significantly decreased by topical laminarin. Additionally, topical laminarin significantly suppressed protein levels of oxazolone-induced proinflammatory cytokines, such as interleukin-1β, tumor necrosis factor-α, monocyte chemoattractant protein-1, and macrophage inflammatory protein-1α in the skin lesion. These results indicate that topical administration of laminarin can alleviate oxazolone-induced atopic dermatitis by inhibiting hyperproduction of IgE, mast cell infiltration, and expressions of proinflammatory cytokines. Based on these findings, we propose that laminarin can be a useful candidate for the treatment of atopic dermatitis.
