Multitarget Phytocomplex: Focus on Antibacterial Profiles of Grape Pomace and <i>Sambucus ebulus</i> L. Lyophilisates Against Extensively Drug-Resistant (XDR) Bacteria and In Vitro Antioxidative Power
Vladimir S. Kurćubić,
Vesna Đurović,
Slaviša B. Stajić,
Marko Dmitrić,
Saša Živković,
Luka V. Kurćubić,
Pavle Z. Mašković,
Jelena Mašković,
Milan Mitić,
Vladimir Živković,
Vladimir Jakovljević
Affiliations
Vladimir S. Kurćubić
Department of Food Technology, Faculty of Agronomy, University of Kragujevac, Cara Dušana 34, 32102 Čačak, Serbia
Vesna Đurović
Department of Biology, Microbiological Biotechnology and Plant Protection, Faculty of Agronomy, University of Kragujevac, Cara Dušana 34, 32102 Čačak, Serbia
Slaviša B. Stajić
Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Belgrade, Serbia
Marko Dmitrić
Veterinary Specialized Institute “Kraljevo”, Žička 34, 36000 Kraljevo, Serbia
Saša Živković
Veterinary Specialized Institute “Kraljevo”, Žička 34, 36000 Kraljevo, Serbia
Luka V. Kurćubić
Department of Medical Microbiology, University Clinical Center of Serbia, Pasterova 2, 11000 Beograd, Serbia
Pavle Z. Mašković
Department of Chemical Engineering, Faculty of Agronomy, University of Kragujevac, Cara Dušana 34, 32102 Čačak, Serbia
Jelena Mašković
Department of Chemical Engineering, Faculty of Agronomy, University of Kragujevac, Cara Dušana 34, 32102 Čačak, Serbia
Milan Mitić
Faculty of Science and Mathematics in Niš, University of Niš, Višegradska 33, 18000 Niš, Serbia
Vladimir Živković
Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, 69 Svetozara Markovica St., 34000 Kragujevac, Serbia
Vladimir Jakovljević
Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, 69 Svetozara Markovica St., 34000 Kragujevac, Serbia
Objectives: This study was conceived with the aim of translating the experience and knowledge of the research group into the design and creation of multi-active phytocomplex cocktails from lyophilised winery by-products (Grape Pomace—GP) and weeds (Sambucus ebulus L., Dwarf Elder—DE). Methods: Quantification of bioactive molecules was performed by high-performance liquid chromatography (HPLC) method. Results: In the extract obtained from lyophilised GP, the most dominant component that was quantified was petunidin-3-glucoside. Prominent compounds that were quantified in DE extract were cyanidin derivatives. The total number of microorganisms in lyophilisates is low, but some of them still survive lyophilisation. Antibacterial activity was determined by microdilution, the minimum inhibitory concentration (MIC) of the tested bacteria ranged from 0.78 mg/mL to 25.00 mg/mL. Antibacterial susceptibility testing (AST) revealed that Klebsiella spp. and Acinetobacter baumannii complex are extensively drug-resistant (XDR). Conclusions: The GP + DE cocktail showed very strong AB power against both tested XDR bacteria. The total phenolic content and antioxidative effect (determined spectrophotometrically) indicate their linear correlation.
