Label-free analysis of adsorbed protein heterogeneity on individual particles, based on single particle collision events

Wei Yi; Cong Xu; Tianyi Xiong; Tienan Gao; Ping Yu; Xiaohua He; Lanqun Mao

Electrochemistry Communications (Feb 2020)

Label-free analysis of adsorbed protein heterogeneity on individual particles, based on single particle collision events

Wei Yi,
Cong Xu,
Tianyi Xiong,
Tienan Gao,
Ping Yu,
Xiaohua He,
Lanqun Mao

Affiliations

Wei Yi: School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China; Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, The Chinese Academy of Sciences (CAS), Beijing 100190, China
Cong Xu: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, The Chinese Academy of Sciences (CAS), Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China
Tianyi Xiong: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, The Chinese Academy of Sciences (CAS), Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China
Tienan Gao: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, The Chinese Academy of Sciences (CAS), Beijing 100190, China
Ping Yu: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, The Chinese Academy of Sciences (CAS), Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China; Corresponding authors at: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, The Chinese Academy of Sciences (CAS), Beijing 100190, China (P. Yu).
Xiaohua He: School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China; Corresponding authors at: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, The Chinese Academy of Sciences (CAS), Beijing 100190, China (P. Yu).
Lanqun Mao: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, The Chinese Academy of Sciences (CAS), Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China

Journal volume & issue: Vol. 111

Abstract

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Investigation of the heterogeneity of protein adsorption on particle surfaces has attracted enormous research attention owing to its great importance in fundamental studies and quality control. Herein we, for the first time, report a new method for label-free analysis of the heterogeneity of protein adsorption on single nanoparticles, based on particle collision events at the orifice of a nanopipette. The dwell time was strongly dependent on the amount of protein adsorbed on polystyrene particles, which could be used to analyze the heterogeneity of protein adsorption at the single particle level. This method presents a label-free, sensitive, reproducible and easily-operated way to analyze adsorption behavior at the single particle level, which opens a new approach to the study of the heterogeneity of physicochemical parameters at the surface of nanoparticles. Keywords: Single particle analysis, Nanopipette, Adsorption heterogeneity, Label-free, Polystyrene particles

Published in Electrochemistry Communications

ISSN: 1388-2481 (Print); 1873-1902 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Technology: Chemical technology: Industrial electrochemistry; Science: Chemistry
Website: https://www.journals.elsevier.com/electrochemistry-communications

About the journal