Molecular characterization of Orf virus isolates from Kodai hills, Tamil Nadu, India

G. Nagarajan; R. Pourouchottamane; G. B. Manjunatha Reddy; R. Yogisharadhya; K. Sumana; S. Rajapandi; G. Murali; S. M. K. Thirumaran; P. K. Mallick; A. S. Rajendiran

doi:10.14202/vetworld.2019.1022-1027

Veterinary World (Jul 2019)

Molecular characterization of Orf virus isolates from Kodai hills, Tamil Nadu, India

G. Nagarajan,
R. Pourouchottamane,
G. B. Manjunatha Reddy,
R. Yogisharadhya,
K. Sumana,
S. Rajapandi,
G. Murali,
S. M. K. Thirumaran,
P. K. Mallick,
A. S. Rajendiran

Affiliations

G. Nagarajan: Southern Regional Research Centre, ICAR-Central Sheep and Wool Research Institute, Kodaikanal, Tamil Nadu, India.
R. Pourouchottamane: ICAR-Central Institute for Research on Goats, Makhdoom, Uttar Pradesh, India.
G. B. Manjunatha Reddy: ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, India.
R. Yogisharadhya: ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, India.
K. Sumana: ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, India.
S. Rajapandi: ICAR-Central Sheep and Wool Research Institute, Avikanagar, Rajasthan, India.
G. Murali: Southern Regional Research Centre, ICAR-Central Sheep and Wool Research Institute, Kodaikanal, Tamil Nadu, India.
S. M. K. Thirumaran: Southern Regional Research Centre, ICAR-Central Sheep and Wool Research Institute, Kodaikanal, Tamil Nadu, India.
P. K. Mallick: ICAR-Central Sheep and Wool Research Institute, Avikanagar, Rajasthan, India.
A. S. Rajendiran: Southern Regional Research Centre, ICAR-Central Sheep and Wool Research Institute, Kodaikanal, Tamil Nadu, India.

DOI: https://doi.org/10.14202/vetworld.2019.1022-1027
Journal volume & issue: Vol. 12, no. 7
pp. 1022 – 1027

Abstract

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Aim: The present study was carried out to find out the causative agent of exanthematous skin lesions in sheep maintained by Southern Regional Research Centre, Mannavanur, Kodai hills, Tamil Nadu. Materials and Methods: Polymerase chain reaction (PCR) with Orf virus (ORFV) B2L gene-specific primers was carried out by employing the total genomic DNA isolated from the scabs as the template. The ORFV isolates from Kodai hills were characterized by the use of bioinformatics tools. Results: The amino acid identity of ORFV isolate 1 from Kodai hills is having 98.14%, 96.29%, and 83.59% identity with reference strains of ORFV, Pseudocowpox virus, and bovine papular stomatitis virus, respectively. Phylogenetic analysis revealed that ORFV isolates from Kodai hills clustered with the other ORFV isolates from different geographical areas of India. Conclusion: The etiological agent of exanthematous skin lesion among sheep of Kodai hills is ORFV.

Published in Veterinary World

ISSN: 0972-8988 (Print); 2231-0916 (Online)
Publisher: Veterinary World
Country of publisher: India
LCC subjects: Agriculture: Animal culture: Veterinary medicine
Website: http://www.veterinaryworld.org

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