Skin Rejuvenation in Aged Mice by Fecal Transplantation Microbiota from Young Mice Feces
Shoujuan Yu,
Ziyang Li,
Xiaoxu Zhang,
Qi Zhang,
Liwei Zhang,
Liang Zhao,
Ping Liu,
Jie Guo,
Juan Chen,
Chengying Zhang,
Xinjuan Liu,
Mengyang Yu,
Dekui Jin,
Xiaofeng Wang,
Guang Li,
Yan Cao,
Fazheng Ren,
Ran Wang
Affiliations
Shoujuan Yu
Key Laboratory of Functional Dairy, Co-Constructed by Ministry of Education and Beijing Government, Department of Nutrition and Health, China Agricultural University, Beijing 100193, China
Ziyang Li
Key Laboratory of Functional Dairy, Co-Constructed by Ministry of Education and Beijing Government, Department of Nutrition and Health, China Agricultural University, Beijing 100193, China
Xiaoxu Zhang
Key Laboratory of Functional Dairy, Co-Constructed by Ministry of Education and Beijing Government, Department of Nutrition and Health, China Agricultural University, Beijing 100193, China
Qi Zhang
Advanced Innovation Center for Food Nutrition and Human Health, Department of Nutrition and Health, China Agricultural University, Beijing 100193, China
Liwei Zhang
Key Laboratory of Functional Dairy, Co-Constructed by Ministry of Education and Beijing Government, Department of Nutrition and Health, China Agricultural University, Beijing 100193, China
Liang Zhao
Research Center for Probiotics, China Agricultural University, Beijing 100083, China
Ping Liu
Research Center for Probiotics, China Agricultural University, Beijing 100083, China
Jie Guo
Key Laboratory of Functional Dairy, Co-Constructed by Ministry of Education and Beijing Government, Department of Nutrition and Health, China Agricultural University, Beijing 100193, China
Juan Chen
Key Laboratory of Functional Dairy, Co-Constructed by Ministry of Education and Beijing Government, Department of Nutrition and Health, China Agricultural University, Beijing 100193, China
Chengying Zhang
Department of General Practice, The Third Centers of Chinese PLA General Hospital, Beijing 100039, China
Xinjuan Liu
Department of Gastroenterology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China
Mengyang Yu
Department of General Practice, The Third Centers of Chinese PLA General Hospital, Beijing 100039, China
Dekui Jin
Department of General Practice, The Third Centers of Chinese PLA General Hospital, Beijing 100039, China
Xiaofeng Wang
Department of Gastroenterology, The Third Centers of Chinese PLA General Hospital, Beijing 100039, China
Guang Li
Department of Gastroenterology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China
Yan Cao
Department of General Practice, The Third Centers of Chinese PLA General Hospital, Beijing 100039, China
Fazheng Ren
Advanced Innovation Center for Food Nutrition and Human Health, Department of Nutrition and Health, China Agricultural University, Beijing 100193, China; Corresponding authors.
Ran Wang
Key Laboratory of Functional Dairy, Co-Constructed by Ministry of Education and Beijing Government, Department of Nutrition and Health, China Agricultural University, Beijing 100193, China; Corresponding authors.
Skin aging is an increasingly prominent topic in the context of healthy aging. During the aging process, the skin’s barrier function diminishes, its water content decreases, wrinkles begin to form, and changes occur in the gut microbiota composition. However, the relationship between gut microbiota and skin aging remains unclear. In this study, we explored skin rejuvenation in aged mice through fecal microbiota transplantation (FMT) using feces from young mice. The results demonstrated enhanced water retention, thickened stratum corneum, increased collagen content, and improved epithelial cell differentiation in aged mice following FMT. Notably, FMT particularly increased the abundance of Lactobacillus and Lactococcus in aged mice, which were nearly undetectable in untreated aged mice. Non-targeted and targeted metabolomics analyses indicated that FMT significantly elevated levels of tryptophan (Trp) and its microbiota metabolites (e.g., indole-3-lactic acid (ILA)) in the feces and serum of aged mice. Both Trp and ILA appeared to rejuvenate aged skin by activating the aryl hydrocarbon receptor (AhR) to promote epidermal cell differentiation. In conclusion, FMT from young mice rejuvenated aged skin via Trp-metabolizing bacteria (Lactobacillus and Lactococcus) and Trp-derived metabolites, suggesting that interventions targeting Trp metabolites may effectively improve skin aging.
