Poultry Science (Feb 2023)
Research Note: Duck plague virus pUL48 is a late protein that plays an important role in viral replication
- Tong Zhou,
- Mingshu Wang,
- Peilin Ruan,
- Dengjian Fan,
- Anchun Cheng,
- Wei Zhang,
- Bin Tian,
- Qiao Yang,
- Ying Wu,
- Shaqiu Zhang,
- Xumin Ou,
- Sai Mao,
- Juan Huang,
- Qun Gao,
- Di Sun,
- Xinxin Zhao,
- Shun Chen,
- Mafeng Liu,
- Dekang Zhu,
- Renyong Jia
Affiliations
- Tong Zhou
- Avian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, China
- Mingshu Wang
- Avian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, China
- Peilin Ruan
- Avian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, China
- Dengjian Fan
- Avian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, China
- Anchun Cheng
- Avian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, China; Corresponding author:
- Wei Zhang
- Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, China
- Bin Tian
- Avian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, China
- Qiao Yang
- Avian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, China
- Ying Wu
- Avian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, China
- Shaqiu Zhang
- Avian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, China
- Xumin Ou
- Avian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, China
- Sai Mao
- Avian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, China
- Juan Huang
- Avian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, China
- Qun Gao
- Avian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, China
- Di Sun
- Avian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, China
- Xinxin Zhao
- Avian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, China
- Shun Chen
- Avian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, China
- Mafeng Liu
- Avian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, China
- Dekang Zhu
- Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, China
- Renyong Jia
- Avian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, China
- Journal volume & issue
-
Vol. 102,
no. 2
p. 102358
Abstract
ABSTRACT: Duck plague virus (DPV) pUL48 is a homologous of herpes simplex virus VP16, and some studies have shown that VP16 is essential for viral replication and proliferation, but there are few studies on DPV pUL48. Therefore, in order to study the function of pUL48 protein, we constructed a UL48-deleted mutant (DPV-BAC-∆UL48) that completely reemoved the UL48 gene from the DPV BAC genome and the revertant virus (DPV-BAC-∆UL48R) by using the 2-step red recombination system. Compared with the parental virus (DPV-BAC) and the revertant virus, the titer of UL48-deleted mutant was reduced by more than 38.2%, and the efficiency of producing infectious virions was significantly reduced. In addition, the average size of plaques produced by UL48-deleted mutant was about 30% smaller than that of the parental and revertant viruses, suggesting that pUL48 protein affected the cell-to-cell transmission of DPV. Finally, pharmacological inhibition assay showed that pUL48 is a late protein of DPV. In this study, we found that UL48, as a late gene, plays an important role in viral replication by affecting the formation of DPV infectious virion, virus cell-to-cell transmission, and viral genome transcription, which may provide some help for the study of the function of DPV pUL48 protein and the prevention and control of DPV.