Antibody identification and analysis of 80 children with positive platelet antibody in initial screening

Abstract

Objective To explore the distribution and types of platelet antibodies in children with positive platelet antibody in initial screening. Methods Blood samples of 80 pediatric patients who applied for platelet transfusion in our hospital from September 2021 to May 2022 and tested positive for platelet antibodies were identified using the PAKPLUS kit for antibody identification, and the distribution of HLA and HPA antibodies were analyzed. Results Among the 80 reactive samples in initial screening, 9 were negative, 71 were positive. Among the 71 positive cases, 1 was HLA-Ⅰantibody positive(1.41%, 1/71), 21 were HPA antibody positive (29.58%, 21/71), and 49 were both HLA-Ⅰ antibody and HPA antibody positive(69.01%, 49/71). Among the 70 HPA positive cases, 23.95% (17/71) had a single HPA antibody, with 18.31% (13/71) of anti GP Ⅱb/Ⅲa, 2.82% (2/71) of anti GP Ⅰa/Ⅱa, 2.82% (2/71) of anti GP Ⅳ and 0% (0/71) of anti GP Ⅰb/Ⅸ, while 74.65% (53/71) presented multiple HPA antibodies. No statistically significant difference was found in antibody distribution among age, gender, transfusion history and disease types. Conclusion HLA-Ⅰ antibody combined with HPA antibody are the main types of platelet antibodies among children with positive platelet antibodies. Anti-GPⅡb/Ⅲa accounted for the largest proportion of HPA antibodies. Antibody distribution is not releted to age, gender, history of blood transfusion and disease types.

Keywords

• hpa antibody
• hla-ⅰ antibody
• pakplus kit