Protocol for design, construction, and selection of genome phage (gPhage) display libraries

Luis Antonio Rodriguez Carnero; André Azevedo Reis Teixeira; Fenny Hui Fen Tang; Andréia Kuramoto; Maria Júlia Manso Alves; Walter Colli; João Carlos Setubal; Edécio Cunha-Neto; Renata Pasqualini; Wadih Arap; Ricardo José Giordano

STAR Protocols (Dec 2021)

Protocol for design, construction, and selection of genome phage (gPhage) display libraries

Luis Antonio Rodriguez Carnero,
André Azevedo Reis Teixeira,
Fenny Hui Fen Tang,
Andréia Kuramoto,
Maria Júlia Manso Alves,
Walter Colli,
João Carlos Setubal,
Edécio Cunha-Neto,
Renata Pasqualini,
Wadih Arap,
Ricardo José Giordano

Affiliations

Luis Antonio Rodriguez Carnero: Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, SP 05508, Brazil; Corresponding author
André Azevedo Reis Teixeira: Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, SP 05508, Brazil; Corresponding author
Fenny Hui Fen Tang: Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, SP 05508, Brazil; Rutgers Cancer Institute of New Jersey, Newark, NJ 07101, USA
Andréia Kuramoto: Heart Institute (InCor), University of São Paulo School of Medicine, São Paulo, SP 05403, Brazil
Maria Júlia Manso Alves: Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, SP 05508, Brazil
Walter Colli: Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, SP 05508, Brazil
João Carlos Setubal: Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, SP 05508, Brazil
Edécio Cunha-Neto: Heart Institute (InCor), University of São Paulo School of Medicine, São Paulo, SP 05403, Brazil; Division of Clinical Immunology and Allergy, University of São Paulo School of Medicine, São Paulo, SP 01246, Brazil; Institute for Investigation in Immunology, University of São Paulo School of Medicine, São Paulo, SP 01246, Brazil
Renata Pasqualini: Rutgers Cancer Institute of New Jersey, Newark, NJ 07101, USA; Division of Cancer Biology, Department of Radiation Oncology, Rutgers New Jersey Medical School, Newark, NJ 07103, USA
Wadih Arap: Rutgers Cancer Institute of New Jersey, Newark, NJ 07101, USA; Division of Hematology/Oncology, Department of Medicine, Rutgers New Jersey Medical School, Newark, NJ 07103, USA
Ricardo José Giordano: Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, SP 05508, Brazil; Institute for Investigation in Immunology, University of São Paulo School of Medicine, São Paulo, SP 01246, Brazil; Corresponding author

Journal volume & issue: Vol. 2, no. 4
p. 100936

Abstract

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Summary: This protocol describes the genomic phage (gPhage) display platform, a large-scale antigen and epitope mapping technique. We constructed a gPhage display peptide library of a eukaryotic organism, Trypanosoma cruzi (causative agent of Chagas disease), to map the antibody response landscape against the parasite. Here, we used an organism with a relatively large but intronless genome, although future applications could include other prevalent or (re)emerging infectious organisms carrying genomes with a limited number of introns.For complete details on the use and execution of this protocol, please refer to Teixeira et al. (2021).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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