Lysine acetylation of major Chlamydia trachomatis antigens

Jelena Mihailovic; Aleksandra Inic-Kanada; Katarina Smiljanic; Elisabeth Stein; Talin Barisani-Asenbauer; Tanja Cirkovic Velickovic

doi:10.1016/j.euprot.2016.01.007

EuPA Open Proteomics (Mar 2016)

Lysine acetylation of major Chlamydia trachomatis antigens

Jelena Mihailovic,
Aleksandra Inic-Kanada,
Katarina Smiljanic,
Elisabeth Stein,
Talin Barisani-Asenbauer,
Tanja Cirkovic Velickovic

Affiliations

Jelena Mihailovic: Center of Excellence for Molecular Food Sciences and Department of Biochemistry, University of Belgrade—Faculty of Chemistry, Belgrade, Serbia
Aleksandra Inic-Kanada: OCUVAC—Center of Ocular Inflammation and Infection, Laura Bassi Centers of Expertise, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria
Katarina Smiljanic: Center of Excellence for Molecular Food Sciences and Department of Biochemistry, University of Belgrade—Faculty of Chemistry, Belgrade, Serbia
Elisabeth Stein: OCUVAC—Center of Ocular Inflammation and Infection, Laura Bassi Centers of Expertise, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria
Talin Barisani-Asenbauer: OCUVAC—Center of Ocular Inflammation and Infection, Laura Bassi Centers of Expertise, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria
Tanja Cirkovic Velickovic: Center of Excellence for Molecular Food Sciences and Department of Biochemistry, University of Belgrade—Faculty of Chemistry, Belgrade, Serbia

DOI: https://doi.org/10.1016/j.euprot.2016.01.007
Journal volume & issue: Vol. 10, no. C
pp. 63 – 69

Abstract

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Chlamydia trachomatis (Ct) is a human pathogen causing trachoma and infertility. We investigated acetylation at lysine residues of chlamydial antigenic proteins: major outer membrane protein (MOMP), 60 kDa chaperonin (chlamydial Hsp60), elongation factor G (EF-G), enolase and the polymorphic membrane proteins PmpB, PmpE and PmpF. 60 kDa chaperonin, EF-G and PmpB showed the highest degree of acetylation. Our data show that important Ct antigens could be post-translationally modified by acetylation of lysine residues at multiple sites. Further studies are needed to investigate total acetylome of Ct and the impact PTMs might have on Ct biology and pathogenicity.

Published in EuPA Open Proteomics

ISSN: 2212-9685 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General): Genetics
Website: http://www.journals.elsevier.com/eupa-open-proteomics/

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