Construction of a complete set of Neisseria meningitidis mutants and its use for the phenotypic profiling of this human pathogen

Abstract

The bacterium Neisseria meningitidis causes life-threatening meningitis and sepsis. Here, Muir et al. construct a complete collection of defined mutants in protein-coding genes of this organism, which they use to identify its essential genome and to shed light on the functions of multiple genes.