Study on Tissue Culture and Rapid Propagation Technology of Ficus formosana

Abstract

【Objective】It aims to establish an efficient propagation technology system of Ficus formosana.【Method】Taking the stem segments of F. formosana as test materials, the effects of different basic media on the induction rate and germination number of stem segments were studied. The effects of different plant growth regulators, activated carbon and their concentration ratio on the proliferation coefficient, rooting rate and growth of cluster buds were studied by orthogonal tests Finally, different transplanting substrates were set up to study the transplanting and domestication of tissue culture seedlings, and the substrates that could improve the survival rate and quality of tissue culture seedlings were screened.【Result】Under the same condition of adding 1.0 mg/L 6-BA, 0.5 mg/L KT and 0.2 mg/L NAA, the induction effect of WPM basic medium on stem segment of F. formosana was better than that of MS basic medium. After 30 days of culture, the induction rate was 97.13% and the germination number was 3.38. The range results of orthogonal tests showed that NAA was the main plant growth regulator affecting the proliferation of cluster buds, followed by 6-BA. The optimum medium for proliferation of cluster bud was WPM+2.0 mg/L 6-BA+0.5 mg/L KT+0.1 mg/L NAA, and the proliferation coefficient reached 5.77 after 60 days of inoculation, with robust cluster buds. Meanwhile, NAA was also the main factor affecting the rooting rate of cluster buds, and there was no significant difference in the effects of IBA and activated carbon on rooting rate. The optimum medium for rooting of cluster bud was WPM+0.1 mg/L IBA+0.1 mg/L NAA+1 g/L activated carbon, the rooting rate reached 100% after 60 days of inoculation, and the tissue culture seedlings were robust with developed roots, not prone to break. The best substrate for tissue culture seedlings was: river sand∶vermiculite∶perlite = 1∶1∶1. After 30 days of transplanting, the survival rate was 97.78% and the plant grew well.【Conclusion】The best rapid propagation system of F. formosanus is : the stem segment is induced by WPM+1.0 mg/L 6-BA+0.5 mg/L KT+0.2 mg/L NAA, the proliferation and rooting of cluster buds are conducted by adding WPM+2.0 mg/L 6-BA+0.5 mg/L KT+0.1 mg/L NAA and WPM+0.1 mg/L IBA+0.1 mg/L NAA+1 g/L activated carbon, the transplanting and domestication are performed in river sand: vermiculit: perlite = 1∶1∶1.

Keywords

• ficus formosana
• tissue culture and rapid propagation
• induction of cluster bud
• test-tube rooting
• transplanting and domestication