Characterisation of haematopoietic stem and progenitor cells in products intended for transplantation

Abstract

Haematopoietic stem and progenitor cells have been successfully used for autologous and al- logeneic transplantations for many years. Quantitative and qualitative laboratory assays allow controlling di erent cellular products during collection, concentration and preservation. The in- formation about cell number, viability, functionality and purity of the gra ensure safe applica- tion and help predict the likelihood of successful engra ment. Almost 50 years of research have disclosed the great heterogeneity of cells that exhibit a haematopoietic di erentiation potential. This will enable the identification of subpopulations within the population of CD34-positive ha- ematopoietic stem cells.

Keywords

• krvotvorne matične celice
• celice CD34+
• celični pripravki
• pretočna citometrija
• CFU-test