Directed evolution of prenylated FMN-dependent Fdc supports efficient in vivo isobutene production

Annica Saaret; Benoît Villiers; François Stricher; Macha Anissimova; Mélodie Cadillon; Reynard Spiess; Sam Hay; David Leys

doi:10.1038/s41467-021-25598-0

Nature Communications (Sep 2021)

Directed evolution of prenylated FMN-dependent Fdc supports efficient in vivo isobutene production

Annica Saaret,
Benoît Villiers,
François Stricher,
Macha Anissimova,
Mélodie Cadillon,
Reynard Spiess,
Sam Hay,
David Leys

Affiliations

Annica Saaret: Department of Chemistry, Manchester Institute for Biotechnology, University of Manchester
Benoît Villiers: Global Bioenergies
François Stricher: Global Bioenergies
Macha Anissimova: Global Bioenergies
Mélodie Cadillon: Global Bioenergies
Reynard Spiess: Department of Chemistry, Manchester Institute for Biotechnology, University of Manchester
Sam Hay: Department of Chemistry, Manchester Institute for Biotechnology, University of Manchester
David Leys: Department of Chemistry, Manchester Institute for Biotechnology, University of Manchester

DOI: https://doi.org/10.1038/s41467-021-25598-0
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 8

Abstract

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Isobutene is a high value gaseous alkene that is widely used as fuel additive and a chemical building block. Here, the authors report an alternative pathway for isobutene bioproduction by directed evolution of prenylated FMN-dependent ferulic acid decarboxylase.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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