Тонкие химические технологии (Dec 2010)
Elaboration of test for enantiomeric purity determination of chiral monomers of polyamide mimetics of nucleic acids
Abstract
An approach for the determination of enantiomeric purity of chiral monomers of α-polyamide mimetics of nucleic acids is described. The test consisted of three steps: synthesis of each monomer as a racemic mixture from DL-Glu or DL-Ala, then selection of an eluent system for direct enantiomers separation on chiral phase by HPLC, and subsequent estimation of the amount of the major enantiomer obtained from L-Ala or L-Glu