mSystems
(Oct 2021)
DNase Treatment Improves Viral Enrichment in Agricultural Soil Viromes
Jackson W. Sorensen,
Laura A. Zinke,
Anneliek M. ter Horst,
Christian Santos-Medellín,
Alena Schroeder,
Joanne B. Emerson
Affiliations
Jackson W. Sorensen
Department of Plant Pathology, University of California, Davis, Davis, California, USA
Laura A. Zinke
Department of Plant Pathology, University of California, Davis, Davis, California, USA
Anneliek M. ter Horst
Department of Plant Pathology, University of California, Davis, Davis, California, USA
Christian Santos-Medellín
Department of Plant Pathology, University of California, Davis, Davis, California, USA
Alena Schroeder
Department of Plant Pathology, University of California, Davis, Davis, California, USA
Joanne B. Emerson
Department of Plant Pathology, University of California, Davis, Davis, California, USA
DOI
https://doi.org/10.1128/mSystems.00614-21
Journal volume & issue
Vol. 6,
no. 5
Abstract
Read online
Viromics is becoming an increasingly popular method for characterizing soil viral communities. DNase treatment of the viral size fraction prior to DNA extraction is meant to reduce contaminating free DNA and is a common step within viromics protocols to ensure that sequences are of viral origin.
WeChat QR code
Close
