DNase Treatment Improves Viral Enrichment in Agricultural Soil Viromes

Jackson W. Sorensen; Laura A. Zinke; Anneliek M. ter Horst; Christian Santos-Medellín; Alena Schroeder; Joanne B. Emerson

doi:10.1128/mSystems.00614-21

mSystems (Oct 2021)

DNase Treatment Improves Viral Enrichment in Agricultural Soil Viromes

Jackson W. Sorensen,
Laura A. Zinke,
Anneliek M. ter Horst,
Christian Santos-Medellín,
Alena Schroeder,
Joanne B. Emerson

Affiliations

Jackson W. Sorensen: Department of Plant Pathology, University of California, Davis, Davis, California, USA
Laura A. Zinke: Department of Plant Pathology, University of California, Davis, Davis, California, USA
Anneliek M. ter Horst: Department of Plant Pathology, University of California, Davis, Davis, California, USA
Christian Santos-Medellín: Department of Plant Pathology, University of California, Davis, Davis, California, USA
Alena Schroeder: Department of Plant Pathology, University of California, Davis, Davis, California, USA
Joanne B. Emerson: Department of Plant Pathology, University of California, Davis, Davis, California, USA

DOI: https://doi.org/10.1128/mSystems.00614-21
Journal volume & issue: Vol. 6, no. 5

Abstract

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Viromics is becoming an increasingly popular method for characterizing soil viral communities. DNase treatment of the viral size fraction prior to DNA extraction is meant to reduce contaminating free DNA and is a common step within viromics protocols to ensure that sequences are of viral origin.

Published in mSystems

ISSN: 2379-5077 (Online)
Publisher: American Society for Microbiology
Country of publisher: United States
LCC subjects: Science: Microbiology
Website: https://journals.asm.org/journal/msystems

About the journal