The B Chromosomes of <i>Prochilodus lineatus</i> (Teleostei, Characiformes) Are Highly Enriched in Satellite DNAs
José Henrique Forte Stornioli,
Caio Augusto Gomes Goes,
Rodrigo Milan Calegari,
Rodrigo Zeni dos Santos,
Leonardo Moura Giglio,
Fausto Foresti,
Claudio Oliveira,
Manolo Penitente,
Fábio Porto-Foresti,
Ricardo Utsunomia
Affiliations
José Henrique Forte Stornioli
Faculty of Sciences, São Paulo State University, Bauru, São Paulo 17033-360, Brazil
Caio Augusto Gomes Goes
Faculty of Sciences, São Paulo State University, Bauru, São Paulo 17033-360, Brazil
Rodrigo Milan Calegari
Faculty of Sciences, São Paulo State University, Bauru, São Paulo 17033-360, Brazil
Rodrigo Zeni dos Santos
Faculty of Sciences, São Paulo State University, Bauru, São Paulo 17033-360, Brazil
Leonardo Moura Giglio
Faculty of Sciences, São Paulo State University, Bauru, São Paulo 17033-360, Brazil
Fausto Foresti
Department of Structural and Functional Biology, Institute of Biosciences, São Paulo State University, Botucatu, São Paulo 18618-689, Brazil
Claudio Oliveira
Department of Structural and Functional Biology, Institute of Biosciences, São Paulo State University, Botucatu, São Paulo 18618-689, Brazil
Manolo Penitente
Mato Grosso State University, Cuiabá, Mato Grosso 78400-000, Brazil
Fábio Porto-Foresti
Faculty of Sciences, São Paulo State University, Bauru, São Paulo 17033-360, Brazil
Ricardo Utsunomia
Department of Genetics, Institute of Biological Sciences and Health, Rural Federal University of Rio de Janeiro, Seropedica, Rio de Janeiro 23897-000, Brazil
B or supernumerary chromosomes are dispensable elements that are widely present in numerous eukaryotes. Due to their non-recombining nature, there is an evident tendency for repetitive DNA accumulation in these elements. Thus, satellite DNA plays an important role in the evolution and diversification of B chromosomes and can provide clues regarding their origin. The characiform Prochilodus lineatus was one of the first discovered fish species bearing B chromosomes, with all populations analyzed so far showing one to nine micro-B chromosomes and exhibiting at least three morphological variants (Ba, Bsm, and Bm). To date, a single satellite DNA is known to be located on the B chromosomes of this species, but no information regarding the differentiation of the proposed B-types is available. Here, we characterized the satellitome of P. lineatus and mapped 35 satellite DNAs against the chromosomes of P. lineatus, of which six were equally located on all B-types and this indicates a similar genomic content. In addition, we describe, for the first time, an entire population without B chromosomes.
