Jichu yixue yu linchuang (Oct 2023)
Propofol promotes angiogenesis in rats with postmenopausal osteoporosis
Abstract
Objective To investigate the impact of propofol on angiogenesis in postmenopausal osteoporosis (PMOP) rats and the role of Wnt/β-catenin signal pathway in this process. Methods Female Wistar rats were randomly grouped into sham operation group, PMOP group (bilateral ovariectomy), groups of propofol 2.5 mg/kg and of 5.0 mg/kg and propofol+Wnt inhibitor group (propofol 2.5 mg/kg + Wnt inhibitor 2 mg/kg), with 10 rats in each. The serum level of bone alkaline phosphatase (BALP), osteoprotegerin (OPG), nuclear factor κB receptor activating factor ligand (RANKL), vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1) was detected by enzyme linked immunosorbent assay (ELISA); bone mineral density (BMD) of femur was measured by dual-energy X-ray absorptiometry; hematoxylin-eosin (HE) staining microscopy was applied to observe pathological changes of bone tissue; the expression of CD31 in bone tissue was detected by immunohistochemistry; Western blot was applied to detect the expression of bone morphogenetic protein-2 (BMP-2), runt-related transcription factor 2 (Runx2), VEGFA, CD31, Wnt2, phosphorylated glycogen synthase kinase-3 (p-GSK-3) and β-catenin in bone tissue. Results Compared with the sham operation group, the serum RANKL level of PMOP group increased, the level of BALP, OPG, VEGF, Ang-1, expression of CD31 and protein level of BMP-2, Runx2, VEGFA, CD31, Wnt2, p-GSK-3, β-catenin all decreased (P<0.05), the bone trabecula was damaged and the medullary cavity was enlarged; compared with the PMOP group, the changes of the above indexes in the propofol groups and the propofol+Wnt inhibitor group were obviously relieved(P<0.05); compared with the propofol 5.0 mg/kg group, the propofol+Wnt inhibitor group showed inhibition in the activation of Wnt/β-catenin signal pathway, and weaken the promotion of propofol on angiogenesis, bone metabolism regulation and bone tissue morphology improvement in PMOP rats(P<0.05). Conclusions Propofol may promote angiogenesis and regulate bone metabolism in PMOP rats by activating Wnt/β-catenin signal pathway as the potential mechanisms involved in anti-PMOP role process.
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