Generation and characterization of three induced pluripotent stem cell lines for modeling coronary artery vasospasm

Ines Ross Tacco; Joseph Olshausen; Tse Yuan Chan; Naima Turbes; Ming-Yow Hung; Chi-Tai Yeh; Patricia K. Nguyen; Karim Sallam; Nazish Sayed; Ian Y. Chen

Stem Cell Research (Feb 2025)

Generation and characterization of three induced pluripotent stem cell lines for modeling coronary artery vasospasm

Ines Ross Tacco,
Joseph Olshausen,
Tse Yuan Chan,
Naima Turbes,
Ming-Yow Hung,
Chi-Tai Yeh,
Patricia K. Nguyen,
Karim Sallam,
Nazish Sayed,
Ian Y. Chen

Affiliations

Ines Ross Tacco: Cardiology Section, Medical Service, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA; Radiology Service, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA; Department of Radiology, Stanford University School of Medicine, Stanford, CA, USA; Stanford Cardiovascular Institute, Stanford University, Stanford, CA, USA
Joseph Olshausen: Cardiology Section, Medical Service, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA; Radiology Service, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA; Department of Radiology, Stanford University School of Medicine, Stanford, CA, USA; Stanford Cardiovascular Institute, Stanford University, Stanford, CA, USA
Tse Yuan Chan: Cardiology Section, Medical Service, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA; Radiology Service, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA; Department of Radiology, Stanford University School of Medicine, Stanford, CA, USA; Stanford Cardiovascular Institute, Stanford University, Stanford, CA, USA
Naima Turbes: Stanford Cardiovascular Institute, Stanford University, Stanford, CA, USA; Division of Vascular Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, CA, USA
Ming-Yow Hung: Division of Cardiology, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan; Division of Cardiology, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, New Taipei City, Taiwan; Taipei Heart Institute, Taipei Medical University, Taipei, Taiwan; Department of Medical Research & Education, Taipei Medical University–Shuang Ho Hospital, New Taipei City, Taiwan
Chi-Tai Yeh: Division of Cardiology, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan; Division of Cardiology, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, New Taipei City, Taiwan; Taipei Heart Institute, Taipei Medical University, Taipei, Taiwan; Department of Medical Research & Education, Taipei Medical University–Shuang Ho Hospital, New Taipei City, Taiwan
Patricia K. Nguyen: Cardiology Section, Medical Service, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA; Stanford Cardiovascular Institute, Stanford University, Stanford, CA, USA
Karim Sallam: Cardiology Section, Medical Service, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA; Stanford Cardiovascular Institute, Stanford University, Stanford, CA, USA
Nazish Sayed: Stanford Cardiovascular Institute, Stanford University, Stanford, CA, USA; Division of Vascular Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, CA, USA
Ian Y. Chen: Cardiology Section, Medical Service, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA; Radiology Service, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA; Department of Radiology, Stanford University School of Medicine, Stanford, CA, USA; Stanford Cardiovascular Institute, Stanford University, Stanford, CA, USA; Corresponding author at: 3801 Miranda Ave, Palo Alto, CA 94304, USA. (I.Y. Chen).

Journal volume & issue: Vol. 82
p. 103644

Abstract

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Coronary artery vasospasm (CAV) is characterized by transient constriction of epicardial coronary arteries leading to angina. Its disease mechanisms are multifactorial but has centered mostly on endothelial dysfunction and smooth muscle hyperreactivity. To facilitate the investigation of these mechanisms in cell culture, we generated and characterized three induced pluripotent stem cell (iPSC) lines from patients with CAV. These lines demonstrated normal morphology and karyotypes, robust expression of pluripotency markers, and ability for tri-lineage differentiation. Further differentiation of these cell lines into endothelial and smooth muscle cells will allow mechanistic investigation of their relative contributions to CAV in cell culture.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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