In Vitro Cultures of Some Medicinal Plant Species (<i>Cistus</i> × <i>incanus</i>, <i>Verbena officinalis</i>, <i>Scutellaria lateriflora</i>, and <i>Scutellaria baicalensis</i>) as a Rich Potential Source of Antioxidants—Evaluation by CUPRAC and QUENCHER-CUPRAC Assays
Michał Dziurka,
Paweł Kubica,
Inga Kwiecień,
Jolanta Biesaga-Kościelniak,
Halina Ekiert,
Shaimaa A. M. Abdelmohsen,
Fatemah F. Al-Harbi,
Diaa O. El-Ansary,
Hosam O. Elansary,
Agnieszka Szopa
Affiliations
Michał Dziurka
The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, ul. Niezapominajek 21, 30-239 Kraków, Poland
Paweł Kubica
Chair and Department of Pharmaceutical Botany, Medical College, Jagiellonian University, Medyczna 9, 30-688 Kraków, Poland
Inga Kwiecień
Chair and Department of Pharmaceutical Botany, Medical College, Jagiellonian University, Medyczna 9, 30-688 Kraków, Poland
Jolanta Biesaga-Kościelniak
The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, ul. Niezapominajek 21, 30-239 Kraków, Poland
Halina Ekiert
Chair and Department of Pharmaceutical Botany, Medical College, Jagiellonian University, Medyczna 9, 30-688 Kraków, Poland
Shaimaa A. M. Abdelmohsen
Physics Department, Faculty of Science, Princess Nourah Bint Abdulrahman University, Saudi Arabia, Riyadh 84428, Saudi Arabia
Fatemah F. Al-Harbi
Physics Department, Faculty of Science, Princess Nourah Bint Abdulrahman University, Saudi Arabia, Riyadh 84428, Saudi Arabia
Diaa O. El-Ansary
Precision Agriculture Laboratory, Department of Pomology, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria 21545, Egypt
Hosam O. Elansary
Plant Production Department, College of Food and Agricultural Sciences, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
Agnieszka Szopa
Chair and Department of Pharmaceutical Botany, Medical College, Jagiellonian University, Medyczna 9, 30-688 Kraków, Poland
Comparative estimations of the antioxidant activity of methanolic extracts from biomasses of different types of in vitro cultures of Cistus × incanus, Verbena officinalis, Scutellaria lateriflora, and S. baicalensis and also from plant raw materials were performed. The antioxidant measurements were based on the modern assays—cupric ion reducing antioxidant capacity (CUPRAC) and quick, easy, new, cheap, and reproducible CUPRAC (QUENCHER-CUPRAC). The total extractable antioxidants (CUPRAC assay) ranged from 10.4 to 49.7 mmol (100 g)−1 of dry weight (DW) expressed as Trolox equivalent antioxidant capacity (TEAC), and the global antioxidant response (QUENCHER-CUPRAC assay) ranged from 16.0 to 79.1 mmol (100 g)−1 DW for in vitro cultures, whereas for plant raw materials the total extractable antioxidants ranged from 20.9 to 69.5 mmol (100 g)−1 DW, and the global antioxidant response ranged from 67.2 to 97.8 mmol (100 g)−1 DW. Finally, the in vitro cultures could be regarded as an antioxidant-rich alternative resource for the pharmaceutical, health food and cosmetics industries.
