Unstable Simple Volatiles and Gas Chromatography-Tandem Mass Spectrometry Analysis of Essential Oil from the Roots Bark of Oplopanax Horridus Extracted by Supercritical Fluid Extraction
Li Shao,
Mei-Hua Bao,
Dong-Sheng Ouyang,
Chong-Zhi Wang,
Chun-Su Yuan,
Hong-Hao Zhou,
Wei-Hua Huang
Affiliations
Li Shao
Department of Human Anatomy, Histology and Embryology, Institute of Neuroscience, Changsha Medical University, Changsha 410219, China
Mei-Hua Bao
Department of Human Anatomy, Histology and Embryology, Institute of Neuroscience, Changsha Medical University, Changsha 410219, China
Dong-Sheng Ouyang
Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, China
Chong-Zhi Wang
Tang Center for Herbal Medicine Research, The Pritzker School of Medicine, University of Chicago, 5841 South Maryland Avenue, MC 4028, Chicago, IL 60637, USA
Chun-Su Yuan
Tang Center for Herbal Medicine Research, The Pritzker School of Medicine, University of Chicago, 5841 South Maryland Avenue, MC 4028, Chicago, IL 60637, USA
Hong-Hao Zhou
Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, China
Wei-Hua Huang
Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, China
Volatile oil from the root bark of Oplopanax horridus is regarded to be responsible for the clinical uses of the title plant as a respiratory stimulant and expectorant. Therefore, a supercritical fluid extraction method was first employed to extract the volatile oil from the roots bark of O. horridus, which was subsequently analyzed by GC/MS. Forty-eight volatile compounds were identified by GC/MS analysis, including (S,E)-nerolidol (52.5%), τ-cadinol (21.6%) and S-falcarinol (3.6%). Accordingly, the volatile oil (100 g) was subjected to chromatographic separation and purification. As a result, the three compounds, (E)-nerolidol (2 g), τ-cadinol (62 mg) and S-falcarinol (21 mg), were isolated and purified from the volatile oil, the structures of which were unambiguously elucidated by detailed spectroscopic analysis including 1D- and 2D-NMR techniques.
