In vitro Study of Biofilm Sensitivity of to the Enzyme Complex Included in Wobenzym

Abstract

Background. Bacterial films are a marker of chronic recurrent infections. Biofilms on mucous membranes block the inflammatory response of the macroorganism, suppressing the activity of immunocytes, and thereby allow microorganisms to reach high concentrations. Currently, research is being conducted to find medications that can act on biofilms. Enzymes, especially their complexes, are substances that can destroy bacterial films. Objective. Еo determine in vitro the sensitivity of bacterial biofilms formed by vaginal microorganisms to the complex of enzymes included in Wobenzym. Materials and methods. The study included 72 clinical isolates of pure microorganism cultures isolated from the vaginal biotope: Gardnerella vaginalis (3), Enterococcus faecalis (9), Escherichia coli (18), Klebsiella pneumoniae (15), Klebsiella aerogenes (3), Lactobacillus crispatus (3), Streptococcus pyogenes (3), Acinetobacter baumanii (3), Staphylococcus aureus (3), Candida albicans (3), Enterococcus faecium (3), Streptococcus agalactiae (3), Lactobacillus acidophilus (3). Bacterial biofilm formation was determined in polystyrene flat-bottom plates using a modified method of Christensen et al. (1985). The tablet form of Wobenzym was used in the study. The tablet shell was washed with saline, the tablet itself was dissolved in 10 ml of 0.9% NaCl and used for in vitro studies. The result was determined using a reader on a spectrophotometer to determine the optical density (OD) of the formed biofilm. It was believed that the drug acted on the bacterial film, reducing the OD by more than three times. Results. An in vitro study revealed clinical isolates of bacteria that formed biofilms of varying severity. Of the 72 clinical bacterial isolates, 38 formed biofilms. A pronounced effect of the complex of enzymes included in Wobenzym on biofilms formed by microorganisms such as A. baumanii, S. aureus, G. vaginalis and E. faecalis was noted. Conclusion. Wobenzym has an effective destructive effect on biofilms formed by various microorganisms, including G. vaginalis, common causative agents of bacterial vaginosis, as well as staphylococci and enterococci, causative agents of aerobic (nonspecific) vaginitis. Conclusion. The drug Wobenzym has an effective destructive effect on biofilms formed by various microorganisms, including Gardnerella vaginalis, common causative agents of bacterial vaginosis, as well as staphylococci and enterococci, causative agents of aerobic (nonspecific vaginitis).

Keywords

• biofilms of microorganisms
• matrix
• enzymes
• wobenzym