Variation of microbial activities and communities in petroleum-contaminated soils induced by the addition of organic materials and bacterivorous nematodes

Jihai Zhou; Yang Wang; Guomin Huang; Chenyang Zhang; Yanmei Ai; Wei Li; Xiaoping Li; Pingjiu Zhang; Jie Zhang; Yongjie Huang; Shoubiao Zhou; Jiyong Zheng

Ecotoxicology and Environmental Safety (Jun 2022)

Variation of microbial activities and communities in petroleum-contaminated soils induced by the addition of organic materials and bacterivorous nematodes

Jihai Zhou,
Yang Wang,
Guomin Huang,
Chenyang Zhang,
Yanmei Ai,
Wei Li,
Xiaoping Li,
Pingjiu Zhang,
Jie Zhang,
Yongjie Huang,
Shoubiao Zhou,
Jiyong Zheng

Affiliations

Jihai Zhou: Collaborative Innovation Center of Recovery and Reconstruction of Degraded Ecosystem in Wanjiang Basin Co-founded by Anhui Province and Ministry of Education, School of Ecology and Environment, Anhui Normal University, Wuhu 241002, China; Collaborative Innovation Center of Southern Modern Forestry, Nanjing Forestry University, Nanjing 210037, China; Correspondence to: Anhui Normal University, Wuhu 241000, China.
Yang Wang: Collaborative Innovation Center of Recovery and Reconstruction of Degraded Ecosystem in Wanjiang Basin Co-founded by Anhui Province and Ministry of Education, School of Ecology and Environment, Anhui Normal University, Wuhu 241002, China
Guomin Huang: Jiangxi Provincial Key Laboratory for Restoration of Degraded Ecosystems & Watershed Ecohydrology, Nanchang Institute of Technology, Nanchang 330099, China
Chenyang Zhang: Collaborative Innovation Center of Recovery and Reconstruction of Degraded Ecosystem in Wanjiang Basin Co-founded by Anhui Province and Ministry of Education, School of Ecology and Environment, Anhui Normal University, Wuhu 241002, China
Yanmei Ai: Collaborative Innovation Center of Recovery and Reconstruction of Degraded Ecosystem in Wanjiang Basin Co-founded by Anhui Province and Ministry of Education, School of Ecology and Environment, Anhui Normal University, Wuhu 241002, China
Wei Li: Jiangxi Provincial Key Laboratory for Restoration of Degraded Ecosystems & Watershed Ecohydrology, Nanchang Institute of Technology, Nanchang 330099, China
Xiaoping Li: Collaborative Innovation Center of Southern Modern Forestry, Nanjing Forestry University, Nanjing 210037, China
Pingjiu Zhang: Collaborative Innovation Center of Recovery and Reconstruction of Degraded Ecosystem in Wanjiang Basin Co-founded by Anhui Province and Ministry of Education, School of Ecology and Environment, Anhui Normal University, Wuhu 241002, China
Jie Zhang: Collaborative Innovation Center of Recovery and Reconstruction of Degraded Ecosystem in Wanjiang Basin Co-founded by Anhui Province and Ministry of Education, School of Ecology and Environment, Anhui Normal University, Wuhu 241002, China
Yongjie Huang: Collaborative Innovation Center of Recovery and Reconstruction of Degraded Ecosystem in Wanjiang Basin Co-founded by Anhui Province and Ministry of Education, School of Ecology and Environment, Anhui Normal University, Wuhu 241002, China
Shoubiao Zhou: Collaborative Innovation Center of Recovery and Reconstruction of Degraded Ecosystem in Wanjiang Basin Co-founded by Anhui Province and Ministry of Education, School of Ecology and Environment, Anhui Normal University, Wuhu 241002, China
Jiyong Zheng: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Institute of Soil and Water Conservation, Northwest A & F University, Yangling 712100, China; Correspondence to: Northwest A & F University, Yangling 712100, China.

Journal volume & issue: Vol. 237
p. 113559

Abstract

Read online

Bacterivorous nematodes are abundant in petroleum-contaminated soils. However, the ecological functions of bacterivorous nematodes and their impacts together with the addition of organic materials on the activity and diversity of microorganisms in petroleum-contaminated soils remain unknown. To assess such effects, six treatments were established in this study, including uncontaminated nematodes-free soil (Control), petroleum-contaminated soil (PC), petroleum-contaminated soil + 5 nematodes per gram dry soil (PCN), and petroleum-contaminated soil + 5 nematodes per gram dry soil + 1% wheat straw (PCNW), or + 1% rapeseed cake (PCNR), or + 1% biochar (PCNB). Results showed that the enzyme activities in the six treatments generally increased firstly and then decreased during the incubation period. Compared with Control, the invertase activity in PCNW, PCNR, and PCNB increased by 80.6%, 313.5%, and 12.4%, respectively, whereas the urease activity in PC, PCN, PCNW, PCNR, and PCNW increased by 1.2%, 25.5%, 124.3%, 105.3%, and 25.5%, respectively. Petroleum pollution, inoculation of bacterivorous nematodes, and the addition of organic materials all significantly boosted the concentrations of phospholipid fatty acids (PLFAs) of soil bacteria, actinobacteria, and total microorganisms, and increased the concentrations of both G+ and G− bacteria PLFAs and the ratio of G−/G+. The concentration of fungi PLFAs and the ratio of fungi to bacteria were significantly higher in PCNW and PCNR than those in other treatments. Overall, adding bacterivorous nematodes and organic materials to the petroleum-contaminated soil significantly improved soil microbial activity and community structure, suggesting that bacterivorous nematodes could be used for the bioremediation in petroleum contaminated soils.

Published in Ecotoxicology and Environmental Safety

ISSN: 0147-6513 (Print); 1090-2414 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Technology: Environmental technology. Sanitary engineering: Environmental pollution; Geography. Anthropology. Recreation: Environmental sciences
Website: https://www.journals.elsevier.com/ecotoxicology-and-environmental-safety

About the journal

Abstract

Keywords