<i>Aegle marmelos</i> Leaf Extract Phytochemical Analysis, Cytotoxicity, In Vitro Antioxidant and Antidiabetic Activities
Wasim Ahmad,
Mohd Amir,
Adil Ahmad,
Abuzer Ali,
Amena Ali,
Shadma Wahab,
Harshita Abul Barkat,
Mohammad Azam Ansari,
Mohammad Sarafroz,
Ayaz Ahmad,
Md. Abul Barkat,
Prawez Alam
Affiliations
Wasim Ahmad
Department of Pharmacy, Mohammad Al-Mana College for Medical Sciences, Dammam 34222, Saudi Arabia
Mohd Amir
Department of Natural Products and Alternative Medicine, College of Clinical Pharmacy, Imam Abdul Rahman Bin Faisal University, Dammam 31441, Saudi Arabia
Adil Ahmad
Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi 110062, India
Abuzer Ali
Department of Pharmacognosy, College of Pharmacy, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Amena Ali
Department of Pharmaceutical Chemistry, College of Pharmacy, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Shadma Wahab
Department of Pharmacognosy, College of Pharmacy, King Khalid University, Abha 61421, Saudi Arabia
Harshita Abul Barkat
Department of Pharmaceutics, College of Pharmacy, University of Hafr Al-Batin, Hafr Al-Batin 39524, Saudi Arabia
Mohammad Azam Ansari
Department of Epidemic Disease Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi Arabia
Mohammad Sarafroz
Department of Pharmaceutical Chemistry, College of Clinical Pharmacy, Imam Abdul Rahman Bin Faisal University, Dammam 31441, Saudi Arabia
Ayaz Ahmad
Department of Pharmacy, Mohammad Al-Mana College for Medical Sciences, Dammam 34222, Saudi Arabia
Md. Abul Barkat
Department of Pharmaceutics, College of Pharmacy, University of Hafr Al-Batin, Hafr Al-Batin 39524, Saudi Arabia
Prawez Alam
Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia
For many years, Aegle marmelos (A. marmelos) has been used medicinally and as a dietary supplement. Despite this, there are minimal research data on A. marmelos phytochemical properties and pharmacological effects. This study aimed to explore the phytoconstituents, cytotoxicity, glucose uptake, and antioxidant and antidiabetic potential of an alcoholic extract of A. marmelos leaf. The cytotoxicity of A. marmelos in HepG2 cells was tested in vitro, and the results revealed that it has strong cytocompatibility and cytoprotective properties. The extract’s antioxidant activities were investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods. Antioxidant potential was shown to be quite impressive. The enzymes α-amylase and α-glycosidase were found to be substantially inhibited by A. marmelos, with IC50 values of 46.21 and 42.07 mg/mL, respectively. In HepG2 cells, A. marmelos significantly reduced ROS levels that were elevated due to high glucose and enhanced glucose consumption (p A. marmelos could be an effective restorative therapy for diabetes and related diseases.
