Time-resolved NMR monitoring of tRNA maturation

Pierre Barraud; Alexandre Gato; Matthias Heiss; Marjorie Catala; Stefanie Kellner; Carine Tisné

doi:10.1038/s41467-019-11356-w

Nature Communications (Jul 2019)

Time-resolved NMR monitoring of tRNA maturation

Pierre Barraud,
Alexandre Gato,
Matthias Heiss,
Marjorie Catala,
Stefanie Kellner,
Carine Tisné

Affiliations

Pierre Barraud: Expression génétique microbienne, UMR 8261, CNRS, Université de Paris, Institut de biologie physico-chimique
Alexandre Gato: Expression génétique microbienne, UMR 8261, CNRS, Université de Paris, Institut de biologie physico-chimique
Matthias Heiss: Department of Chemistry, Ludwig Maximilians University Munich
Marjorie Catala: Expression génétique microbienne, UMR 8261, CNRS, Université de Paris, Institut de biologie physico-chimique
Stefanie Kellner: Department of Chemistry, Ludwig Maximilians University Munich
Carine Tisné: Expression génétique microbienne, UMR 8261, CNRS, Université de Paris, Institut de biologie physico-chimique

DOI: https://doi.org/10.1038/s41467-019-11356-w
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 14

Abstract

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Transfer RNA (tRNA) is regulated by RNA modifications. Here the authors employ time-resolved NMR to monitor modifications of yeast tRNAPhe in cellular extracts, revealing a sequential order and cross-talk between modifications.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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