Investigation on the teratogenic and embryotoxic effects of nanozeolite on chick embryos model

Abstract

Objective(s): In veterinary medicine, zeolites are used as toxin binder in animal feed, ammonia purification of aquatic pools, animal smell and moisture control. This study investigated on the risk assessment of nano zeolite on development of embryonic chicken models, anomalies, tetratogenic and embryotoxic effects. Methods: Eggs (120 = n) were accidentally divided into 4 groups. In the experimental groups, 0.3 ml of the solution of nanozeolite (5,50,100 mg/L) injected into egg albumin, The eggs were then incubated for 19 days at 60% humidity and 37.5 ° C. At the end of incubation time, the fetus and organs (liver, heart, brain, spleen) weight and congenital anomalies were investigated. Results: The administration of nano-zeolite in chick embryos as a model for evaluating human embryonic damage showed teratogenic effects including deformity of legs and wings, liver and heart disformation at the doses of 50,100 mg/L. The embryo were smaller and significant morphological anomalous changes were observed. The comparison between the three experimental groups showed that the dose of 5 mg/L improved viability of chicken and showed increasing the dose of zeolite increases teratogenic effects and increased fetal mortality rate. Conclusions: The teratogenic effects of nanozeolits on chick embryo should be considered in the risk assessment of nanoparticles on human embryo and fetous.

Keywords

• nanoparticles
• nanozeolite
• chicken embryo
• embryotoxic
• teratogenic